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QTRT1 Protein (AA 1-403) (His tag)
QTRT1
Origine: Humain
Hôte: Escherichia coli (E. coli)
Recombinant
> 80 % by SDS - PAGE
SDS
N° du produit ABIN6387595
Détail du produit
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Antigène
Voir toutes QTRT1 Protéines
QTRT1
(Queuine tRNA-Ribosyltransferase 1 (QTRT1))
Type de proteíne
Recombinant
Attributs du protein
AA 1-403
Origine
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Humain
Source
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Escherichia coli (E. coli)
Purification/Conjugué
Cette QTRT1 protéine est marqué à la His tag.
Application
SDS-PAGE (SDS)
Séquence
MGSSHHHHHH SSGLVPRGSH MGSEFMAGAA TQASLESAPR IMRLVAECSR SRARAGELWL PHGTVATPVF MPVGTQATMK GITTEQLDAL GCRICLGNTY HLGLRPGPEL IQKANGLHGF MNWPHNLLTD SGGFQMVSLV SLSEVTEEGV RFRSPYDGNE TLLSPEKSVQ IQNALGSDII MQLDDVVSST VTGPRVEEAM YRSIRWLDRC IAAHQRPDKQ NLFAIIQGGL DADLRATCLE EMTKRDVPGF AIGGLSGGES KSQFWRMVAL STSRLPKDKP RYLMGVGYAT DLVVCVALGC DMFDCVFPTR TARFGSALVP TGNLQLRKKV FEKDFGPIDP ECTCPTCQKH SRAFLHALLH SDNTAALHHL TVHNIAYQLQ LMSAVRTSIV EKRFPDFVRD FMGAMYGDPT LCPTWATDAL ASVGITLG
Pureté
> 80 % by SDS - PAGE
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Information d'application
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Indications d'application
Optimal working dilution should be determined by the investigator.
Restrictions
For Research Use only
Stockage
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Format
Liquid
Concentration
0.25 mg/mL
Buffer
Liquid. In Phosphate buffered saline ( pH 7.4) containing 30 % glycerol, 1 mM DTT
Stock
4 °C,-20 °C,-80 °C
Stockage commentaire
Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
Détail du antigène
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Antigène
QTRT1
(Queuine tRNA-Ribosyltransferase 1 (QTRT1))
Autre désignation
QTRT1 (QTRT1 Produits )
Synonymes
CG4947 Protein, Dmel\\CG4947 Protein, TGT Protein, tgt Protein, zgc:66378 Protein, FP3235 Protein, TGUT Protein, 2610028E17Rik Protein, Tgt Protein, Tgut Protein, tRNA-guanine transglycosylase Protein, queuine tRNA-ribosyltransferase 1 S homeolog Protein, queuine tRNA-ribosyltransferase 1 Protein, queuine tRNA-ribosyltransferase catalytic subunit 1 Protein, Tgt Protein, Olsu_0732 Protein, Palpr_1298 Protein, Riean_1227 Protein, Calni_1240 Protein, Ftrac_0312 Protein, Ocepr_1541 Protein, Sulku_0977 Protein, Tmar_0854 Protein, Bache_2323 Protein, Nitsa_1291 Protein, Isop_1657 Protein, Despr_0021 Protein, Pedsa_1842 Protein, Corgl_1148 Protein, Mahau_0921 Protein, Theth_0831 Protein, qtrt1.S Protein, qtrt1 Protein, QTRT1 Protein, Qtrt1 Protein
Sujet
QTRT1 also known as queuine tRNA-ribosyltransferase. QTRT1 interacts with QTRTD1 to form an active queuine tRNA-ribosyltransferase. This enzyme exchanges queuine for the guanine at the wobble position of tRNAs with GUN anticodons (tRNA-Asp, -Asn, -His and -Tyr), thereby forming the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine). Recombinant QTRT1 was expressed in E.coli and purified by using conventional chromatography techniques.
Poids moléculaire
46.7kDa (428aa) confirmed by MALDI-TOF
NCBI Accession
NP_112486
UniProt
Q9BXR0
Pathways
Ribonucleoside Biosynthetic Process
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