NME4 Protein (AA 33-187) (His tag)

N° du produit ABIN6387712

Détail du produit

Antigène: NME4 (NME/NM23 Nucleoside Diphosphate Kinase 4 (NME4))

Type de proteíne: Recombinant

Activité biologique: Active

Attributs du protein: AA 33-187

Origine: Humain

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette NME4 protéine est marqué à la His tag.

Application: SDS-PAGE (SDS)

Séquence: MGSSHHHHHH SSGLVPRGSH MPSWTRERTL VAVKPDGVQR RLVGDVIQRF ERRGFTLVGM KMLQAPESVL AEHYQDLRRK PFYPALIRYM SSGPVVAMVW EGYNVVRASR AMIGHTDSAE AAPGTIRGDF SVHISRNVIH ASDSVEGAQR EIQLWFQSSE LVSWADGGQH SSIHPA

Pureté: > 90 % by SDS - PAGE

Biological Activity Comment: Specific activity is > 120 units/mg, and is defined as the amount of enzyme that convert 1.0 umole each of ATP and TDP to ADP and TTP per minute at pH 7.5 at 25C in a couple system with PK/LDH.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

Bioactivity Validated

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Liquid. 20  mM Tris-HCl buffer (  pH 8.0) containing 40 % glycerol, 0.2M NaCl

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Détail du antigène

Antigène: NME4 (NME/NM23 Nucleoside Diphosphate Kinase 4 (NME4))

Autre désignation: NME4 (NME4 Produits)

Synonymes: NDPK-D Protein, NM23H4 Protein, nm23-H4 Protein, 2610027N22Rik Protein, 2810024O08Rik Protein, 5730493H09Rik Protein, NM23-M4 Protein, Nm23M4 Protein, NME/NM23 nucleoside diphosphate kinase 4 Protein, NME4 Protein, Nme4 Protein

Sujet: NME4, also known as nucleoside diphosphate kinase, mitochondrial, belongs to the NDK family. NME4 are ubiquitous enzymes that catalyze transfer of gamma-phosphates, via a phosphohistidine intermediate, between nucleoside and dioxynucleoside tri- and diphosphates. The enzymes are products of the nm23 gene family, which includes NME4. NME4 plays a major role in the synthesis of nucleoside triphosphates other than ATP. Recombinant human NME4 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Poids moléculaire: 19.6 kDa (176aa), confirmed by MALDI-TOF

NCBI Accession: NP_005000

UniProt: O00746

Pathways: Nucleotide Phosphorylation, Ribonucleoside Biosynthetic Process