NME3 Protein (AA 22-169) (His tag)
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- Antigène Voir toutes NME3 Protéines
- NME3 (NME/NM23 Nucleoside Diphosphate Kinase 3 (NME3))
- Type de proteíne
- Recombinant
- Activité biologique
- Active
- Attributs du protein
- AA 22-169
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Origine
- Humain
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Source
- Escherichia coli (E. coli)
- Purification/Conjugué
- Cette NME3 protéine est marqué à la His tag.
- Application
- SDS-PAGE (SDS)
- Séquence
- MGSSHHHHHH SSGLVPRGSH MERTFLAVKP DGVQRRLVGE IVRRFERKGF KLVALKLVQA SEELLREHYA ELRERPFYGR LVKYMASGPV VAMVWQGLDV VRTSRALIGA TNPADAPPGT IRGDFCIEVG KNLIHGSDSV ESARREIALW FRADELLCWE DSAGHWLYE
- Pureté
- > 95 % by SDS - PAGE
- Biological Activity Comment
- Specific activity is > 150 units/mg, and is defined as the amount of enzyme that convert 1.0 umole each of ATP and TDP to ADP and TTP per minute at pH 7.5 at 25C in a couple system with PK/LDH.
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- Discover our top product NME3 Protéine
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NME/NM23 Nucleoside Diphosphate Kinase 3 (NME3) (AA 22-169) protein (His tag)
NME3 Origine: Humain Hôte: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
NME/NM23 Nucleoside Diphosphate Kinase 3 (NME3) (AA 1-169) protein (GST tag)NME3 Origine: Humain Hôte: Wheat germ Recombinant WB, ELISA, AP, AA
NME/NM23 Nucleoside Diphosphate Kinase 3 (NME3) (AA 73-169) protein (GST tag)NME3 Origine: Humain Hôte: Wheat germ Recombinant WB, ELISA, AP, AA
NME/NM23 Nucleoside Diphosphate Kinase 3 (NME3) (AA 1-169) protein (Strep Tag)Crystallography grade NME3 Origine: Humain Hôte: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
NME/NM23 Nucleoside Diphosphate Kinase 3 (NME3) (AA 1-169) protein (Strep Tag)Crystallography grade NME3 Origine: Souris Hôte: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
NME/NM23 Nucleoside Diphosphate Kinase 3 (NME3) proteinNME3 Origine: Souris Hôte: Escherichia coli (E. coli) Recombinant > 95 % SDS, WB, ELISA, IP
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- Indications d'application
- Optimal working dilution should be determined by the investigator.
- Commentaires
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Bioactivity Validated
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Liquid. 20 mM Tris-HCl buffer ( pH 8.0) containing 50 % glycerol, 0.1M NaCl, 2 mM DTT
- Stock
- 4 °C,-20 °C,-80 °C
- Stockage commentaire
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Antigène
- NME3 (NME/NM23 Nucleoside Diphosphate Kinase 3 (NME3))
- Autre désignation
- NME3 (NME3 Produits)
- Synonymes
- DR-nm23 Protein, NDPK-C Protein, NDPKC Protein, NM23-H3 Protein, NM23H3 Protein, c371H6.2 Protein, 1810009F08Rik Protein, AI413736 Protein, Ndk3 Protein, Nm23-DR Protein, Nm23-M3 Protein, NME/NM23 nucleoside diphosphate kinase 3 Protein, NME3 Protein, Nme3 Protein
- Sujet
- NME3, also known as, a potential suppressor of metastasis, is expressed at a much lower level in highly metastatic cells than in cells with lower metastatic potential. It is important for the synthesis of nucleoside triphosphates and may play a role in apoptosis induction and hematopoiesis. It is preferentially expressed during early stages of myeloid differentiation of highly purified CD34+ cells. Recombinant human NME3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
- Poids moléculaire
- 19.1 kDa (169aa), confirmed by MALDI-TOF
- NCBI Accession
- NP_002504
- UniProt
- Q13232
- Pathways
- Nucleotide Phosphorylation, Ribonucleoside Biosynthetic Process
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