PLAP Protein (AA 23-506) (His tag)

N° du produit ABIN7529299

Détail du produit

Antigène: PLAP (ALPP) (Placental Alkaline Phosphatase (ALPP))

Type de proteíne: Recombinant

Activité biologique: Active

Attributs du protein: AA 23-506

Origine: Humain

Source: Baculovirus infected Insect Cells

Purification/Conjugué: Cette PLAP protéine est marqué à la His tag.

Application: SDS-PAGE (SDS)

Séquence: ADLMIIPVEE ENPDFWNREA AEALGAAKKL QPAQTAAKNL IIFLGDGMGV STVTAARILK GQKKDKLGPE LPLAMDRFPY VALSKTYNVD KHVPDSGATA TAYLCGVKGN FQTIGLSAAA RFNQCNTTRG NEVISVMNRA KKAGKSVGVV TTTRVQHASP AGTYAHTVNR NWYSDADVPA SARQEGCQDI ATQLISNMDI DVILGGGRKY MFRMGTPDPE YPDDYSQGGT RLDGKNLVQE WLAKRQGARY VWNRTELMQA SLDPSVTHLM GLFEPGDMKY EIHRDSTLDP SLMEMTEAAL RLLSRNPRGF FLFVEGGRID HGHHESRAYR ALTETIMFDD AIERAGQLTS EEDTLSLVTA DHSHVFSFGG YPLRGSSIFG LAPGKARDRK AYTVLLYGNG PGYVLKDGAR PDVTESESGS PEYRQQSAVP LDEETHAGED VAVFARGPQA HLVHGVQEQT FIAHVMAFAA CLEPYTACDL APPAGTTDHH HHHH

Pureté: > 95 % by SDS - PAGE

niveau d'endotoxine: < 1.0 EU per 1 microgram of protein (determined by LAL method)

Biological Activity Comment: Specific activity is > 2,500 units/mg, and is defined as the amount of enzyme that hydrolyze 1.0 nmole of p-nitrophenyl phosphate (pNPP) per minute at pH 7.5 at 37C.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

Bioactivity Validated

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Liquid. In Phosphate Buffered Saline (  pH 7.4) containing 10 % glycerol.

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Détail du antigène

Antigène: PLAP (ALPP) (Placental Alkaline Phosphatase (ALPP))

Autre désignation: Alkaline phosphatase, placental (ALPP Produits)

Synonymes: ECK0378 Protein, JW0374 Protein, psiA Protein, ALP Protein, PALP Protein, PLAP Protein, PLAP-1 Protein, ILC Protein, CTACK Protein, ESkine Protein, Akp2 Protein, alp Protein, zgc:56672 Protein, DDBDRAFT_0205491 Protein, DDBDRAFT_0231570 Protein, DDB_0205491 Protein, DDB_0231570 Protein, ALPI Protein, Actn2lp Protein, Alp Protein, bacterial alkaline phosphatase Protein, alkaline phosphatase, placental Protein, alkaline phosphatase, placental type Protein, C-C motif chemokine ligand 27 Protein, alkaline phosphatase, liver/bone/kidney Protein, alkaline phosphatase Protein, alkaline phosphatase, intestinal Protein, PDZ and LIM domain 3 Protein, phoA Protein, ALPP Protein, LOC100436725 Protein, CCL27 Protein, alpl Protein, alp Protein, ALPI Protein, Pdlim3 Protein, Alpp Protein

Sujet: ALPP, also known as alkaline phosphatase, placental, is a family of dimeric metalloenzymes catalyzing the hydrolysis of monoesters of phosphoric acid. Also, this protein catalyzes a transphosphorylation reaction in the presence of large concentrations of phosphate acceptors. It occurs widely in nature, and are found in many organisms from Escherichia coli to man. Most alkaline phosphatases are homodimeric enzymes and each catalytic site contains three metal ions (two Zn and one Mg), that are necessary for enzymatic activity. Recombinant human ALPP, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Poids moléculaire: 53.9kDa (494aa) 50-70KDa (SDS-PAGE under reducing conditions).