GLO1 Protein (AA 1-184) (His tag)
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- Antigène Voir toutes GLO1 Protéines
- GLO1 (Glyoxalase I (GLO1))
- Type de proteíne
- Recombinant
- Activité biologique
- Active
- Attributs du protein
- AA 1-184
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Origine
- Souris
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Source
- Baculovirus infected Insect Cells
- Purification/Conjugué
- Cette GLO1 protéine est marqué à la His tag.
- Application
- SDS-PAGE (SDS)
- Séquence
- MAEPQPASSG LTDETAFSCC SDPDPSTKDF LLQQTMLRIK DPKKSLDFYT RVLGLTLLQK LDFPAMKFSL YFLAYEDKND IPKDKSEKTA WTFSRKATLE LTHNWGTEDD ETQSYHNGNS DPRGFGHIGI AVPDVYSACK RFEELGVKFV KKPDDGKMKG LAFIQDPDGY WIEILNPNKI ATIILEHHHH HH
- Pureté
- > 95 % by SDS - PAGE
- niveau d'endotoxine
- < 1.0 EU per 1 microgram of protein (determined by LAL method)
- Biological Activity Comment
- Specific activity is > 210 units/mg, and is defined as the amount of enzyme that will form 1.0 micromol of S-lactoylglutathione from methylglyoxal and reduced glutathione per minute at pH 6.5 at 25C.
- Top Product
- Discover our top product GLO1 Protéine
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- Indications d'application
- Optimal working dilution should be determined by the investigator.
- Commentaires
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Bioactivity Validated
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Liquid. In Phosphate Buffered Saline ( pH 7.4) containing 10 % glycerol.
- Stock
- 4 °C,-20 °C,-80 °C
- Stockage commentaire
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Antigène
- GLO1 (Glyoxalase I (GLO1))
- Autre désignation
- Glo1 (GLO1 Produits)
- Synonymes
- GLOD1 Protein, GLYI Protein, 0610009E22Rik Protein, 1110008E19Rik Protein, 2510049H23Rik Protein, AW550643 Protein, GLY1 Protein, Glo-1 Protein, Glo-1r Protein, Glo-1s Protein, Glo1-r Protein, Glo1-s Protein, Qglo Protein, cb554 Protein, zgc:66035 Protein, wu:fb82g09 Protein, glod1 Protein, glyi Protein, glo1 Protein, GLO1 Protein, ATGLX1 Protein, F12F1.32 Protein, F12F1_32 Protein, GLYOXALASE I Protein, glyoxalase I homolog Protein, trypanothione-dependent glyoxalase I Protein, glyoxalase I Protein, glyoxalase 1 Protein, glyoxalase 1 L homeolog Protein, glyoxalase 1 S homeolog Protein, lactoylglutathione lyase Protein, glyoxalase/bleomycin resistance protein/dioxygenase superfamily protein Protein, GLO1 Protein, Glo1 Protein, glo1 Protein, glo1.L Protein, glo1.S Protein, GST3 Protein, GLX1 Protein, STY1687 Protein, Bcen_2094 Protein, gloA Protein
- Sujet
- Glo1, also known as lactoylglutathione lyase, is a member of the glyoxalase l family. It plays a critical role in the detoxification of 2-oxoaldehydes, such as methylglyoxal. It involved in the regulation of TNF-induced transcriptional activity of NF-kappa-B. It was identified as a protein marker, which is consistently expressed to a higher extent in LAB-M than in HAB-M mice in several brain areas. Recombinant mouse Glo1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
- Poids moléculaire
- 21.8kDa (192aa) 28-40kDa (SDS-PAGE under reducing conditions)
- NCBI Accession
- NP_079650
- UniProt
- Q9CPU0
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