GDA Protein (AA 1-454) (His tag)

N° du produit ABIN7281157

Détail du produit

Antigène: GDA (Guanine Deaminase (GDA))

Type de proteíne: Recombinant

Activité biologique: Active

Attributs du protein: AA 1-454

Origine: Humain

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette GDA protéine est marqué à la His tag.

Application: SDS-PAGE (SDS)

Séquence: MGSSHHHHHH SSGLVPRGSH METGPEDPSS MPEESSPRRT PQSIPYQDLP HLVNADGQYL FCRYWKPTGT PKALIFVSHG AGEHSGRYEE LARMLMGLDL LVFAHDHVGH GQSEGERMVV SDFHVFVRDV LQHVDSMQKD YPGLPVFLLG HSMGGAIAIL TAAERPGHFA GMVLISPLVL ANPESATTFK VLAAKVLNLV LPNLSLGPID SSVLSRNKTE VDIYNSDPLI CRAGLKVCFG IQLLNAVSRV ERALPKLTVP FLLLQGSADR LCDSKGAYLL MELAKSQDKT LKIYEGAYHV LHKELPEVTN SVFHEINMWV SQRTATAGTA SPP

Pureté: > 85 % by SDS - PAGE

Biological Activity Comment: Specific activity is >170 units/mg, and is defined as the amount of enzyme that hydrolyze 1.0 umole of p-nitrophenyl butyrate to p-nitrophenol per minute at pH 7.5 at 25C.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

Bioactivity Validated

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Liquid. In 20 mM Tris-HCl Buffer (  pH 8.0) containing 10 % Glycerol

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Détail du antigène

Antigène: GDA (Guanine Deaminase (GDA))

Autre désignation: GDA (GDA Produits)

Synonymes: CYPIN Protein, GUANASE Protein, NEDASIN Protein, AU015411 Protein, AW047581 Protein, Cypin Protein, MGC81835 Protein, zgc:112282 Protein, AGR_C_4212 Protein, DDBDRAFT_0169373 Protein, DDBDRAFT_0230211 Protein, DDB_0169373 Protein, DDB_0230211 Protein, guanine deaminase Protein, guanine deaminase L homeolog Protein, short-chain dehydrogenase/reductase sdr Protein, GDA Protein, Gda Protein, gda.L Protein, gda Protein, SPO2956 Protein, Gdia_3192 Protein, Mchl_2046 Protein, Mnod_4655 Protein, guaD Protein, Dd586_1771 Protein, Kvar_2512 Protein, Mrub_1462 Protein, BC1002_2263 Protein, Cseg_1079 Protein, Mesil_1795 Protein, Trad_2850 Protein, Mesop_0346 Protein, gDA Protein, ML5_2802 Protein

Sujet: MGLL is membrane-associated member of the serine hydrolase superfamily. MGLL functions together with hormone-sensitive lipase (LIPE) to hydrolyze intracellular triglyceride stores in adipocytes and other cells to fatty acids and glycerol. MGLL may also complement lipoprotein lipase (LPL) in completing hydrolysis of monoglycerides resulting from degradation of lipoprotein triglycerides. It is most abundantly expressed in skeletal muscle and adipose tissue. Recombinant human MGLL protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Poids moléculaire: 36.4 kDa(333aa), confirmed by MALDI-TOF

NCBI Accession: NP_009214

UniProt: Q99685