GLUL Protein (AA 1-373) (His tag)

N° du produit ABIN6387882

Détail du produit

Antigène: GLUL (Glutamate-Ammonia Ligase (GLUL))

Type de proteíne: Recombinant

Activité biologique: Active

Attributs du protein: AA 1-373

Origine: Humain

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette GLUL protéine est marqué à la His tag.

Application: SDS-PAGE (SDS)

Séquence: MGSSHHHHHH SSGLVPRGSH MSGPRPVVLS GPSGAGKSTL LKRLLQEHSG IFGFSVSHTT RNPRPGEENG KDYYFVTREV MQRDIAAGDF IEHAEFSGNL YGTSKVAVQA VQAMNRICVL DVDLQGVRNI KATDLRPIYI SVQPPSLHVL EQRLRQRNTE TEESLVKRLA AAQADMESSK EPGLFDVVII NDSLDQAYAE LKEALSEEIK KAQRTGA

Pureté: > 90 % by SDS - PAGE

Biological Activity Comment: Specific activity is >100 units/mg and is defined as the amount of enzyme that convert 1.0 umole of GMP and ATP to GDP and ADP per minute at pH 7.5 at 37C in coupled system with PK/LDH.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

Bioactivity Validated

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid. In 20  mM Tris-HCl buffer(  pH 8.0) containing 10 % glycerol, 1  mM DTT, 0.1M NaCl

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Détail du antigène

Antigène: GLUL (Glutamate-Ammonia Ligase (GLUL))

Autre désignation: GLUL (GLUL Produits)

Synonymes: GLNS Protein, GS Protein, PIG43 Protein, PIG59 Protein, Glns Protein, glns Protein, glul Protein, pig43 Protein, pig59 Protein, xgs Protein, p42 Protein, MGC64262 Protein, cb195 Protein, cb757 Protein, etID49849.2 Protein, fb39g09 Protein, fd19d09 Protein, gso1 Protein, sb:cb195 Protein, wu:fb39g09 Protein, wu:fd19d09 Protein, fb11d08 Protein, gso2 Protein, hm:zehn1949 Protein, im:7153900 Protein, wu:fb11d08 Protein, glutamate-ammonia ligase Protein, glutamate-ammonia ligase (glutamine synthetase) Protein, type I glutamate--ammonia ligase Protein, glutamate-ammonia ligase L homeolog Protein, glutamate-ammonia ligase (glutamine synthase) Protein, glutamate-ammonia ligase (glutamine synthase) a Protein, glutamate-ammonia ligase (glutamine synthase) b Protein, GLUL Protein, Glul Protein, AMF_RS09530 Protein, glul.L Protein, glul Protein, CNA04370 Protein, glula Protein, glulb Protein

Sujet: GUK1, also known as GMK, belongs to the guanylate kinase family. This protein exists as a monomer that catalyzes the ATP-dependent conversion of GMP to GDP, thereby playing an essential role in the recycling of GMP. Via its catalytic activity, GUK1 is thought to participate in regulating the supply of guanine nucleotides to signal transduction pathways. Overexpression of GUK1 is associated with pituitary adenocarcinomas, suggesting that GUK1 is involved in tumorigenesis. Recombinant human GUK1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Poids moléculaire: 23.9 kDa (217aa), confirmed by MALDI-TOF

NCBI Accession: NP_000849

UniProt: Q16774

Pathways: Positive Regulation of Peptide Hormone Secretion