ENO2/NSE Protein (AA 1-434) (His tag)

N° du produit ABIN7281198

Détail du produit

Antigène: ENO2/NSE (ENO2) (Enolase 2 (Gamma, Neuronal) (ENO2))

Type de proteíne: Recombinant

Activité biologique: Active

Attributs du protein: AA 1-434

Origine: Souris

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette ENO2/NSE protéine est marqué à la His tag.

Application: SDS-PAGE (SDS)

Séquence: MGSSHHHHHH SSGLVPRGSH MGSMSIEKIW AREILDSRGN PTVEVDLYTA KGLFRAAVPS GASTGIYEAL ELRDGDKQRY LGKGVLKAVD HINSRIAPAL ISSGISVVEQ EKLDNLMLEL DGTENKSKFG ANAILGVSLA VCKAGAAERD LPLYRHIAQL AGNSDLILPV PAFNVINGGS HAGNKLAMQE FMILPVGAES FRDAMRLGAE VYHTLKGVIK DKYGKDATNV GDEGGFAPNI LENSEALELV KEAIDKAGYT EKMVIGMDVA ASEFYRDGKY DLDFKSPADP SRYITGDQLG ALYQDFVRNY PVVSIEDPFD QDDWAAWSKF TANVGIQIVG DDLTVTNPKR IERAVEEKAC NCLLLKVNQI GSVTEAIQAC KLAQENGWGV MVSHRSGETE DTFIADLVVG LCTGQIKTGA PCRSERLAKY NQLMRIEEEL GDEARFAGHN FRNPSVL

Pureté: > 95 % by SDS - PAGE

Biological Activity Comment: Specific activity is > 10,000 pmol/min/ug, and was obtained by measuring the decrease of NAD in absorbance at 340nm resulting from NADH at pH 6.5 at 37C.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid. In Phosphate Buffered Saline (  pH 7.4) containing 10 % glycerol

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Détail du antigène

Antigène: ENO2/NSE (ENO2) (Enolase 2 (Gamma, Neuronal) (ENO2))

Autre désignation: Eno2 (ENO2 Produits)

Synonymes: ENO2 Protein, DKFZp459B1817 Protein, NSE Protein, AI837106 Protein, D6Ertd375e Protein, Eno-2 Protein, RNEN3 Protein, eno3 Protein, wu:fc09h05 Protein, zgc:92418 Protein, enolase 2 Protein, enolase 2 (gamma, neuronal) Protein, enolase 2, gamma neuronal Protein, enolase 2, gamma, neuronal Protein, ENO2 Protein, Eno2 Protein, eno2 Protein

Sujet: Eno2, also known as Gamma enolase isoform1, is a glycolytic isoenzyme which is located in central and peripheral neurons and neuroendocrine cells. This enzyme is released into the CSF when neural tissue is injured. Neoplasms derived from neural or neuroendocrine tissue may release Eno2 into the blood. Eno2 is a useful substance that has been detected in patients with certain tumors, namely: neuroblastoma, small cell lung cancer, medullary thyroid cancer, carcinoid tumors, pancreatic endocrine tumors, and melanoma. Recombinant mouse Eno2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Poids moléculaire: 49.7kDa (457aa) confirmed by MALDI-TOF

NCBI Accession: NP_038537

UniProt: P17183