PIN1 Protein (AA 1-165) (His tag)

N° du produit ABIN6388010

Détail du produit

Antigène: PIN1 (Peptidylprolyl Cis/trans Isomerase, NIMA-Interacting 1 (PIN1))

Type de proteíne: Recombinant

Activité biologique: Active

Attributs du protein: AA 1-165

Origine: Souris

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette PIN1 protéine est marqué à la His tag.

Application: SDS-PAGE (SDS)

Séquence: MGSSHHHHHH SSGLVPRGSH MGSMADEEKL PPGWEKRMSR SSGRVYYFNH ITNASQWERP SGGSTVGGSS KNGQGEPAKV RCSHLLVKHS QSRRPSSWRQ EKITRSKEEA LELINGYIQK IKSGEEDFES LASQFSDCSS AKARGDLGPF SRGQMQKPFE DASFALRTGE MSGPVFTDSG IHIILRTE

Pureté: > 95 % by SDS - PAGE

niveau d'endotoxine: < 1.0 EU per 1 microgram of protein (determined by LAL method)

Biological Activity Comment: Specific activity is > 1,200 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmole of suc-AAFP-PNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

Bioactivity Validated

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid. In Phosphate buffered saline (  pH 7.4) containing 10 % glycerol

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Détail du antigène

Antigène: PIN1 (Peptidylprolyl Cis/trans Isomerase, NIMA-Interacting 1 (PIN1))

Autre désignation: Pin1 (PIN1 Produits)

Synonymes: DOD Protein, UBL5 Protein, dod Protein, ubl5 Protein, pin1 Protein, pin1a Protein, 0610025L01Rik Protein, D9Bwg1161e Protein, pin1b Protein, zgc:73206 Protein, PPIase pin1 Protein, MdPin1 Protein, Pin1 Protein, peptidylprolyl cis/trans isomerase, NIMA-interacting 1 Protein, peptidylprolyl cis/trans isomerase, NIMA-interacting 1 S homeolog Protein, protein (peptidyl-prolyl cis/trans isomerase) NIMA-interacting 1 Protein, peptidylprolyl cis/trans isomerase, NIMA-interacting 1 L homeolog Protein, peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 Protein, peptidyl-prolyl cis-trans isomerase Pin1 Protein, PIN1 Protein, Pin1 Protein, pin1 Protein, pin1.S Protein, pin1.L Protein, ppiD Protein, PITG_11123 Protein, LOC103419188 Protein

Sujet: Pin1, also known as peptidyl-prolyl cis-trans isomerase NIMA-interacting1, is nuclear PPIase containing a WW protein interaction domain, and is structurally and functionally related to Ess1/Ptf1, an essential protein in budding yeast. Pin1 is thus an essential PPIase that regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity. Substrates of Pin1 include the mitotic regulators (Cdc25 phosphatase and NIMA, PLK I, Wee, and Myt1 kinases), several transcription factors like beta-Catenin, c-Jun, and the tumor suppressor protein p53 , and some specific proteins like the RNA Pol II, the cytoskeleton protein tau, and the G1/S protein Cyclin D1. Recombinant mouse PIN1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Poids moléculaire: 20.8 kDa (188aa) Confirmed by MALDI-TOF

NCBI Accession: NP_075860

UniProt: Q9QUR7