ECHS1 Protein (AA 28-290) (His tag)

N° du produit ABIN6388011

Détail du produit

Antigène: ECHS1 (Enoyl CoA Hydratase, Short Chain, 1, Mitochondrial (ECHS1))

Type de proteíne: Recombinant

Activité biologique: Active

Attributs du protein: AA 28-290

Origine: Humain

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette ECHS1 protéine est marqué à la His tag.

Application: SDS-PAGE (SDS)

Séquence: MGSSHHHHHH SSGLVPRGSH MASGANFEYI IAEKRGKNNT VGLIQLNRPK ALNALCDGLI DELNQALKIF EEDPAVGAIV LTGGDKAFAA GADIKEMQNL SFQDCYSSKF LKHWDHLTQV KKPVIAAVNG YAFGGGCELA MMCDIIYAGE KAQFAQPEIL IGTIPGAGGT QRLTRAVGKS LAMEMVLTGD RISAQDAKQA GLVSKICPVE TLVEEAIQCA EKIASNSKIV VAMAKESVNA AFEMTLTEGS KLEKKLFYST FATDDRKEGM TAFVEKRKAN FKDQ

Pureté: > 95 % by SDS - PAGE

Biological Activity Comment: Specific activity is > 150 units/mg, and is defined as the amount of enzyme that hydrolyze 1.0 umole of crotonoyl-CoA to hydroxybutyryl-CoA per minute per minute at pH 7.5 at 25C.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

Bioactivity Validated

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid. 20  mM Tris-HCl buffer (  pH 8.0) containing 1  mM DTT, 20 % glycerol, 100  mM NaCl

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Détail du antigène

Antigène: ECHS1 (Enoyl CoA Hydratase, Short Chain, 1, Mitochondrial (ECHS1))

Autre désignation: ECHS1 (ECHS1 Produits)

Synonymes: SCEH Protein, cOR6not Protein, fj55e05 Protein, si:zc217g15.1 Protein, wu:fj55e05 Protein, C80529 Protein, enoyl-CoA hydratase, short chain, 1, mitochondrial L homeolog Protein, enoyl-CoA hydratase, short chain 1 Protein, enoyl CoA hydratase, short chain, 1, mitochondrial Protein, enoyl Coenzyme A hydratase, short chain, 1, mitochondrial Protein, echs1.L Protein, ECHS1 Protein, Echs1 Protein, echs1 Protein

Sujet: Enoyl Coenzyme A hydratase, short chain 1 mitochondrial, also known as ECHS1, is a member of the hydratase/isomerase superfamily. It localizes to the mitochondrial matrix. Expressed in muscle, liver and fibroblasts, with low expression in kidney and spleen, ECHS1 exists as a homohexamer that functions in the second step of the mitochondrial fatty acid beta-oxidation pathway. Recombinant human ECHS1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Poids moléculaire: 30.6 kDa (284aa), confirmed by MALDI-TOF

Pathways: Monocarboxylic Acid Catabolic Process