AHCY Protein (AA 1-432) (His tag)

N° du produit ABIN6388020

Détail du produit

Antigène: AHCY (Adenosylhomocysteinase (AHCY))

Type de proteíne: Recombinant

Attributs du protein: AA 1-432

Origine: Humain

Source: Baculovirus infected Insect Cells

Purification/Conjugué: Cette AHCY protéine est marqué à la His tag.

Application: SDS-PAGE (SDS)

Séquence: ADLMSDKLPY KVADIGLAAW GRKALDIAEN EMPGLMRMRE RYSASKPLKG ARIAGCLHMT VETAVLIETL VTLGAEVQWS SCNIFSTQDH AAAAIAKAGI PVYAWKGETD EEYLWCIEQT LYFKDGPLNM ILDDGGDLTN LIHTKYPQLL PGIRGISEET TTGVHNLYKM MANGILKVPA INVNDSVTKS KFDNLYGCRE SLIDGIKRAT DVMIAGKVAV VAGYGDVGKG CAQALRGFGA RVIITEIDPI NALQAAMEGY EVTTMDEACQ EGNIFVTTTG CIDIILGRHF EQMKDDAIVC NIGHFDVEID VKWLNENAVE KVNIKPQVDR YRLKNGRRII LLAEGRLVNL GCAMGHPSFV MSNSFTNQVM AQIELWTHPD KYPVGVHFLP KKLDEAVAEA HLGKLNVKLT KLTEKQAQYL GMSCDGPFKP DHYRYHHHHH H

Pureté: > 90 % by SDS - PAGE

niveau d'endotoxine: < 1.0 EU per 1 microgram of protein (determined by LAL method)

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: Liquid. In Phosphate Buffered Saline (  pH 7.4) containing 10 % glycerol.

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Détail du antigène

Antigène: AHCY (Adenosylhomocysteinase (AHCY))

Autre désignation: AHCY (AHCY Produits)

Synonymes: sahh Protein, PSPTO5068 Protein, Ahcy Protein, SAHH Protein, adoHcyase Protein, ahcy Protein, AA987153 Protein, AL024110 Protein, CuBP Protein, D150 Protein, cb1079 Protein, hm:zeh1173 Protein, hm:zeh1364 Protein, wu:fj67b02 Protein, adenosylhomocysteinase Protein, Adenosylhomocysteinase Protein, S-adenosylhomocysteine hydrolase Protein, adenosylhomocysteinase S homeolog Protein, AHCY Protein, ahcy Protein, ahcY Protein, MMAH_RS02605 Protein, Srot_2217 Protein, Ndas_3755 Protein, Deba_0936 Protein, Palpr_2392 Protein, Calni_0569 Protein, LOC100282150 Protein, Intca_2510 Protein, Marky_0392 Protein, Desac_1394 Protein, Tc00.1047053511229.50 Protein, Tc00.1047053511589.200 Protein, Tb11.01.1350 Protein, LMJF_36_3910 Protein, MCYG_06244 Protein, Ahcy Protein, ahcy.S Protein

Sujet: AHCY, also known as adenosylhomocysteinase isoform 1, is an enzyme that catalyzes the reversible hydrolysis of S-adenosylhomocysteine (AdoHcy) to adenosine (Ado) and L-homocysteine (Hcy). AdoHcy hydrolysis is a reversible reaction with an equilibrium favoring AdoHcy formation, but hydrolysis prevails under physiological conditions due to the rapid removal of adenosine and homocysteine. Thus, AHCYs activity in mammals is directly related to homocysteine level, an independent risk factor for vascular disease. It also functions as a regulator of biological transmethylation by controlling the concentration of AdoHcy, a potent competitive inhibitor of all S-adenosyl-L-methionine methyltransferases. Recombinant human AHCY protein, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Poids moléculaire: 48.8kDa (441aa) 40-57KDa (SDS-PAGE under reducing conditions.)

NCBI Accession: NP_000678

UniProt: P23526