FGF4 Protéine

N° du produit ABIN6699858

N° du produit (Fournisseur): 009-001-u76-0005

Protéine Recombinant FGF4 exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour FGF4 Protéine (ABIN6699858)

Antigène: FGF4 (Fibroblast Growth Factor 4 (FGF4))

Type de proteíne: Recombinant

Origine: Humain

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Pureté: 98,00%

Détail du produit

N° du produit (Fournisseur): 009-001-u76-0005

Fournisseur: Rockland

Fonction: Human Fibroblast Growth Factor-4 Recombinant Protein

Purification: Fibroblast Growth Factor-4 purity was determined to be greater than 98% as determined by analysis by reducing and non-reducing SDS-pAGE.

niveau d'endotoxine: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity is determined by its ability to induce the proliferation of mouse NR6R-3T3 fibroblasts and is typically 0.25-1.25 ng/mL.

Information d'application

Indications d'application:

Application Note: Fibroblast Growth Factor-4 Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-Fibroblast Growth Factor-4 in immunological assays. Lyophilized in 10 mM sodium phosphate, 75 mM sodium chloride, pH 7.5.

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 5 μL (5-50 μL)

Buffer: Lyophilized in 10 mM sodium phosphate, 75 mM sodium chloride, pH 7.5.

Agent conservateur: Without preservative

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Date de péremption: 6 months

Détail du antigène

Antigène: FGF4 (Fibroblast Growth Factor 4 (FGF4))

Autre désignation: FGF4

Sujet:

Synonyms: Transforming protein KS3, Heparin-binding growth factor (HBGF-4), Heparin secretory-transforming protein 1 (HST-1)

Background: Fibroblast Growth Factor 4 (FGF-4) is a growth factor predominantly expressed during embryonic development, playing a key role in limb development. In culture, FGF-4 has been shown to be an important regulator of growth for stem cells, fibroblasts and endothelial cells. Unglycosylated FGF-4 is N-terminally cleaved into 13 kDa or 15 kDa proteins that are more active than the precursor 19 kDa protein, Bellosa P, et al. (1993). Human FGF-4 shares high homology and cross-reactivity with the mouse protein. Recombinant human FGF-4 is a non-glycosylated protein containing 140 amino acids, with a total molecular weight of 15 kDa.

UniProt: P08620

Pathways: Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance