KIT Ligand Protein (KITLG)

N° du produit ABIN6700197

N° du produit (Fournisseur): 010-001-w20-0100

Protéine Recombinant KIT Ligand exprimée dans Escherichia coli (E. coli) et a été mentionnée dans 1 publication.

Aperçu rapide pour KIT Ligand Protein (KITLG) (ABIN6700197)

Antigène: KIT Ligand (KITLG)

Type de proteíne: Recombinant

Origine: Souris

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Pureté: 98,00%

Détail du produit

N° du produit (Fournisseur): 010-001-w20-0100

Fournisseur: Rockland

Fonction: Mouse Stem Cell Factor Recombinant Protein

Purification: Stem Cell Factor purity was determined to be greater than 98% as determined by analysis by UV-Spectroscopy at 280nm and by reducing and non-reducing SDS-pAGE.

niveau d'endotoxine: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity is determined by the dose-dependent proliferation of human TF-1 cells and is typically less than 10 ng/mL.

Information d'application

Indications d'application:

Application Note: Stem Cell Factor Recombinant Protein has been tested by biological activity and is suitable as a control for polyclonal or monoclonal anti-Stem Cell Factor in immunological assays. Lyophilized with 20 mM sodium phosphate, pH 6.5.

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 100 μL

Concentration: 0.1 mg/mL

Buffer: Lyophilized with 20 mM sodium phosphate, pH 6.5.

Agent conservateur: Without preservative

Stock: -20 °C

Stockage commentaire: Store vial at -20° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Date de péremption: 6 months

Référence

Turnis, Kaminska, Smith, Kartchner, Vogel, Laxton, Ashmun, Ney, Opferman: "Requirement for antiapoptotic MCL-1 during early erythropoiesis." dans: Blood, Vol. 137, Issue 14, pp. 1945-1958, (2021) (PubMed).

Détail du antigène

Antigène: KIT Ligand (KITLG)

Autre désignation: Kitlg

Sujet:

Synonyms: c-Kit Ligand, Hematopoietic growth factor KL, KL, Steel Factor, Stem cell factor (SCF), Mast cell growth factor(MGF)

Background: Stem Cell Factor (SCF) is a cytokine made by fibroblasts and endothelial cells. SCF binds to the receptor known as c-Kit (CD117) and is thought to play a critical role in the maintenance or survival of hematopoietic stem cells. Human SCF shows no activity on murine cells, but murine and rat SCF are active on human cells. Recombinant mouse SCF is a non-glycosylated protein, containing 164 amino acids, with a molecular weight of 18.3 kDa.

UniProt: P20826

Pathways: Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway