PDGFB Protéine

N° du produit ABIN6700401

N° du produit (Fournisseur): 009-001-w12-0100

Protéine Recombinant PDGFB exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour PDGFB Protéine (ABIN6700401)

Antigène: PDGFB (Platelet Derived Growth Factor Subunit B (PDGFB))

Type de proteíne: Recombinant

Origine: Humain

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Pureté: 95,00%

Détail du produit

N° du produit (Fournisseur): 009-001-w12-0100

Fournisseur: Rockland

Fonction: Human Platelet Derived Growth Factor-BB Recombinant Protein

Purification: platelet Derived Growth Factor-BB purity was determined to be greater than 95% as determined by analysis by UV-Spectroscopy at 280nm and by reducing and non-reducing SDS-pAGE.

niveau d'endotoxine: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity is determined by the dose-dependent proliferation of 3T3 indicator cells and is typically 1-3 ng/mL.

Information d'application

Indications d'application:

Application Note: Platelet Derived Growth Factor-BB Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-Platelet Derived Growth Factor-BB in immunological assays.

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 100 μL

Concentration: 0.1 mg/mL

Buffer:

Buffer: 0.01 M Sodium Phosphate, pH 7.5

Stabilizer: None

Agent conservateur: Without preservative

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Date de péremption: 6 months

Détail du antigène

Antigène: PDGFB (Platelet Derived Growth Factor Subunit B (PDGFB))

Autre désignation: PDGFB

Sujet:

Synonyms: PDGF-2, Platelet-derived growth factor B chain, Platelet-derived growth factor beta polypeptide, Proto-oncogene c-Sis

Background: Platelet-Derived Growth Factor (PDGF) is a mitogenic peptide growth hormone carried in the alpha-granules of platelets and is released when platelets adhere to traumatized tissues. Connective tissue cells near the traumatized region respond by initiating the process of replication. The synthesis of PDGF can be induced by IL-1, IL-6, TNF-α, TGF-β and EGF. Recombinant human PDGF-BB is a non-glycosylated, disulfide-linked homodimer, containing two 109 amino acid chains, with a total molecular weight of 24.3 kDa.

UniProt: P01127

Pathways: Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling