SARS-CoV S1 Protein (His tag)

N° du produit ABIN6952622

Détail du produit

Antigène: SARS-CoV S1 (SARS-Coronavirus Spike Subunit S1 (SARS-CoV S1))

Type de proteíne: Recombinant

Origine: SARS Coronavirus (SARS-CoV)

Source: HEK-293 Cells

Purification/Conjugué: Cette SARS-CoV S1 protéine est marqué à la His tag.

Application: SDS-PAGE (SDS), ELISA

Fonction: SARS S1 protein, His Tag (MALS verified)

Séquence: AA 14-667

Attributs du produit: SARS S1 protein, His Tag is expressed from human 293 cells (HEK293). It contains AA Ser 14 - Arg 667 (Accession # AAP13567.1). Predicted N-terminus: Ser 14
This protein carries a polyhistidine tag at the C-terminus.

Pureté: >95 % as determined by SDS-PAGE.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 1.0 EU per μg by the LAL method.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: PBS, pH 7.4

Conseil sur la manipulation: Please avoid repeated freeze-thaw cycles.

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: For long term storage, the product should be stored at lyophilized state at -20°C or lower.
This product is stable after storage at:
4-8°C for 12 months in lyophilized state,
-70°C for 3 years under sterile conditions after reconstitution.

Référence

Kondo, Iwatani, Matsuoka, Fujino, Umemoto, Yokomaku, Ishizaki, Kito, Sezaki, Hayashi, Murakami: "Antibody-like proteins that capture and neutralize SARS-CoV-2." dans: Science advances, Vol. 6, Issue 42, (2020) (PubMed).

Tosif, Neeland, Sutton, Licciardi, Sarkar, Selva, Do, Donato, Quan Toh, Higgins, Van de Sandt, Lemke, Lee, Shoffner, Flanagan, Arnold, Mordant, Mulholland, Bines, Dohle, Pellicci, Curtis, McNab et al.: "Immune responses to SARS-CoV-2 in three children of parents with symptomatic COVID-19. ..." dans: Nature communications, Vol. 11, Issue 1, pp. 5703, (2020) (PubMed).

Détail du antigène

Antigène: SARS-CoV S1 (SARS-Coronavirus Spike Subunit S1 (SARS-CoV S1))

Autre désignation: SARS-Coronavirus Spike Subunit S1 (SARS-CoV S1 Produits)

Classe de substances: Viral Protein

Sujet: It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Poids moléculaire: 74.9 kDa