SARS-CoV Spike Protein (RBD) (His tag)

N° du produit ABIN6952627

Détail du produit

Antigène: SARS-CoV Spike (SARS-CoV S) (SARS-Coronavirus Spike Protein (SARS-CoV S))

Type de proteíne: Recombinant

Attributs du protein: RBD

Origine: SARS Coronavirus (SARS-CoV)

Source: HEK-293 Cells

Purification/Conjugué: Cette SARS-CoV Spike protéine est marqué à la His tag.

Application: ELISA, SDS-PAGE (SDS)

Fonction: SARS S protein RBD, His Tag (MALS verified)

Séquence: AA 306-527

Attributs du produit: SARS S protein RBD, His Tag is expressed from human 293 cells (HEK293). It contains AA Arg 306 - Phe 527 (Accession # AAP13567.1). Predicted N-terminus: Arg 306
This protein carries a polyhistidine tag at the C-terminus.

Pureté: > 90 % as determined by SDS-PAGE.
> 95 % as determined by SEC-MALS.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 1.0 EU per μg by the LAL method.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: PBS, pH 7.4

Conseil sur la manipulation: Please avoid repeated freeze-thaw cycles.

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: For long term storage, the product should be stored at lyophilized state at -20°C or lower.
This product is stable after storage at:
4-8°C for 12 months in lyophilized state,
-70°C for 3 years under sterile conditions after reconstitution.

Référence

Longworth, Dittmar: "An antigen microarray protocol for COVID-19 serological analysis." dans: STAR protocols, Vol. 2, Issue 3, pp. 100815, (2021) (PubMed).

Détail du antigène

Antigène: SARS-CoV Spike (SARS-CoV S) (SARS-Coronavirus Spike Protein (SARS-CoV S))

Autre désignation: SARS-Coronavirus Spike Protein (SARS-CoV S Produits)

Classe de substances: Viral Protein

Sujet: It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Poids moléculaire: 26.9 kDa