SARS-CoV-2 NSP3 Protein (His tag)

N° du produit ABIN6952636

Détail du produit

Antigène: SARS-CoV-2 NSP3 (NSP3) (SARS-CoV-2 Papain-Like Protease (NSP3))

Type de proteíne: Recombinant

Origine: SARS Coronavirus-2 (SARS-CoV-2)

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette SARS-CoV-2 NSP3 protéine est marqué à la His tag.

Application: SDS-PAGE (SDS)

Fonction: SARS-CoV-2 (COVID-19) Papain-like Protease Protein, His Tag

Séquence: AA 746-1060

Attributs du produit: SARS-CoV-2 Papain-like Protease, His Tag is expressed from E.coli cells. It contains AA Glu 746 - Lys 1060 (Accession # YP_009725299.1). Predicted N-terminus: Met
This protein carries a polyhistidine tag at the N-terminus.

Pureté: >95 % as determined by SDS-PAGE.

Stérilité: 0.2 μm filtered

niveau d'endotoxine: Less than 1.0 EU per μg by the LAL method.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 50 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH 7.5

Conseil sur la manipulation: Please avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: This product is stable after storage at:
The product MUST be stored at -70°C or lower upon receipt,
-70°C for 3 months under sterile conditions.

Référence

Kim, Seiler, Jones, Ridout, Camp, Fabrizio, Jeevan, Miller, Throm, Ferrara, Fredrickson, Lowe, Wang, Odemuyiwa, Wan, Webby: "Antibody Responses to SARS-CoV-2 Antigens in Humans and Animals." dans: Vaccines, Vol. 8, Issue 4, (2020) (PubMed).

Détail du antigène

Antigène: SARS-CoV-2 NSP3 (NSP3) (SARS-CoV-2 Papain-Like Protease (NSP3))

Abstract: NSP3 Produits

Synonymes: pp1ab Protein, ORF1a polyprotein;ORF1ab polyprotein Protein, ORF1ab Protein

Classe de substances: Viral Protein

Sujet: New coronavirus is a single-stranded RNA positive-strand envelope type B coronavirus. Similar to the other two coronaviruses that cause SARS (Severe Acute Respiratory Syndrome) and MERS (Middle East Respiratory Syndrome), its genome encodes non-structural, structural, and accessory proteins. Non-structural proteins include 3-chymotrypsin-like protease (3CLpro), papain-like protease, helicase, and RNA-dependent RNA polymerase (RNA -dependent RNA polymerase (RdRp). Structural proteins include spike glycoproteins. Papain in coronavirus is the same as 3CLpro. This enzyme operates on no less than 11 cleavage sites on the large polyprotein 1ab. Processing of polyproteins translated from viral RNA is essential. Therefore, press this The activity of this enzyme will prevent virus imitation.

Poids moléculaire: 37.6 kDa

NCBI Accession: YP_009725299