SARS-CoV-2 NSP12 (RdRP) Protein (His tag)

N° du produit ABIN6952695

Détail du produit

Antigène: SARS-CoV-2 NSP12 (RdRP) (SARS-CoV-2 RdRP) (SARS-CoV-2 RNA-dependent RNA Polymerase (NSP12) (SARS-CoV-2 RdRP))

Type de proteíne: Recombinant

Origine: SARS Coronavirus-2 (SARS-CoV-2)

Source: Cellules d'insectes

Purification/Conjugué: Cette SARS-CoV-2 NSP12 (RdRP) protéine est marqué à la His tag.

Application: Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Séquence: MSADAQSFLNR VCGVSAARLT PCGTGTSTDV VYRAFDIYND KVAGFAKFLK TNCCRFQEKD EDDNLIDSYF VVKRHTFSNY QHEETIYNLL KDCPAVAKHD FFKFRIDGDM VPHISRQRLT KYTMADLVYA LRHFDEGNCD TLKEILVTYN CCDDDYFNKK DWYDFVENPD ILRVYANLGE RVRQALLKTV QFCDAMRNAG IVGVLTLDNQ DLNGNWYDFG DFIQTTPGSG VPVVDSYYSL LMPILTLTRA LTAESHVDTD LTKPYIKWDL LKYDFTEERL KLFDRYFKYW DQTYHPNCVN CLDDRCILHC ANFNVLFSTV FPPTSFGPLV RKIFVDGVPF VVSTGYHFRE LGVVHNQDVN LHSSRLSFKE LLVYAADPAM HAASGNLLLD KRTTCFSVAA LTNNVAFQTV KPGNFNKDFY DFAVSKGFFK EGSSVELKHF FFAQDGNAAI SDYDYYRYNL PTMCDIRQLL FVVEVVDKYF DCYDGGCINA NQVIVNNLDK SAGFPFNKWG KARLYYDSMS YEDQDALFAY TKRNVIPTIT QMNLKYAISA KNRARTVAGV SICSTMTNRQ FHQKLLKSIA ATRGATVVIG TSKFYGGWHN MLKTVYSDVE NPHLMGWDYP KCDRAMPNML RIMASLVLAR KHTTCCSLSH RFYRLANECA QVLSEMVMCG GSLYVKPGGT SSGDATTAYA NSVFNICQAV TANVNALLST DGNKIADKYV RNLQHRLYEC LYRNRDVDTD FVNEFYAYLR KHFSMMILSD DAVVCFNSTY ASQGLVASIK NFKSVLYYQN NVFMSEAKCW TETDLTKGPH EFCSQHTMLV KQGDDYVYLP YPDPSRILGA GCFVDDIVKT DGTLMIERFV SLAIDAYPLT KHPNQEYADV FHLYLQYIRK LHDELTGHML DMYSVMLTND NTSRYWEPEF YEAMYTPHTV LQHHHHHH

Attributs du produit:

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Commentaires:

C-Terminal His-Tag

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 50 mM Hepes, pH 8; 1000 mM NaCl; 1 % Glycerin, 3 mM MgCl2 and protease inhibitors

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: SARS-CoV-2 NSP12 (RdRP) (SARS-CoV-2 RdRP) (SARS-CoV-2 RNA-dependent RNA Polymerase (NSP12) (SARS-CoV-2 RdRP))

Autre désignation: SARS-CoV-2 RNA-directed RNA Polymerase (SARS-CoV-2 RdRP Produits)

Synonymes: pp1ab Protein, ORF1a polyprotein;ORF1ab polyprotein Protein, ORF1ab Protein

Classe de substances: Viral Protein

Sujet: Cleavage product of rpp1ab (AA 5393-5324) polyprotein from the Wuhan-Hu-1 isolate, Wuhan, China Dec 2019
Responsible for replication and transcription of the viral RNA genomeB

Poids moléculaire: 106661

NCBI Accession: YP_009725307

UniProt: P0DTD1