SARS-CoV-2 Nucleocapsid Protein (SARS-CoV-2 N) (G204R, R203K) (His tag)

N° du produit ABIN6992418

Détail du produit

Antigène: SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N)

Type de proteíne: Recombinant

Activité biologique: Active

Attributs du protein: G204R, R203K

Origine: SARS Coronavirus-2 (SARS-CoV-2)

Source: HEK-293 Cells

Purification/Conjugué: Cette SARS-CoV-2 Nucleocapsid protéine est marqué à la His tag.

Fonction: SARS-CoV-2 Nucleocapsid protein (R203K, G204R), His Tag

Séquence: AA 1-419

Attributs du produit: SARS-CoV-2 Nucleocapsid protein (R203K, G204R), His Tag is expressed from human 293 cells (HEK293). It contains AA Met 1 - Ala 419 (Accession # QHO62115.1 (R203K, G204R). The nucleocapsid protein is consisted of the N-terminal RNA-binding domain (NTD) and the C-terminal dimerization domain (CTD), divided by a central serine/arginine-rich (SR)-linker responsible for phosphorylation. The mutations (R203K, G204R) were identified on the SR-linker on the nucleocapsid protein of SARS-CoV-2 variants. Phosphorylation of this SR-link motif in SARS-CoV modulates nucleocapsid multimerization, translational inhibitory activity and cellular localization. The co-occuring mutations R203K and G204R may decrease the overall structural flexibility of SARS-COV-2 N protein.

Pureté: >90 % as determined by SDS-PAGE.

niveau d'endotoxine: Less than 1.0 EU per μg by the LAL method.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: PBS, pH 7.4

Stock: -20 °C

Détail du antigène

Antigène: SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N)

Autre désignation: SARS-CoV-2 Nucleocapsid protein (SARS-CoV-2 N Produits)

Synonymes: nucleocapsid phosphoprotein Protein, N Protein

Classe de substances: Viral Protein

Sujet: Nucleocapsid (N) protein is the most abundant protein found in coronavirus. CoV N protein is a highly immunogenic phosphoprotein important for viral genome replication and modulation of cell signaling pathways. It was first identified by a research team while they were screening for ADP-ribosylated proteins during coronavirus (CoV) infection (Grunewald M. E., et al. 2017, Virology, 517: 62-68). The array of diverse functional activities accommodated in N protein makes it more than a structural protein but also an interesting target in the development of antiviral therapeutics. Because of the conservation of N protein sequence and its strong immunogenicity, N protein of coronavirus is chosen as a diagnostic tool.

Poids moléculaire: 47.4 kDa