SARS-CoV-2 Spike S1 Protein (B.1.617.2 - delta) (His tag,AVI tag,Biotin)

N° du produit ABIN7013339

Détail du produit

Antigène: SARS-CoV-2 Spike S1

Type de proteíne: Recombinant

Activité biologique: Active

Attributs du protein: B.1.617.2 - delta

Origine: SARS Coronavirus-2 (SARS-CoV-2), SARS CoV-2 Delta

Source: HEK-293 Cells

Purification/Conjugué: Cette SARS-CoV-2 Spike S1 protéine est marqué à la His tag,AVI tag,Biotin.

Fonction: Biotinylated SARS-CoV-2 Spike S1 (T19R, G142D, EF156-157del, R158G, L452R, T478K, D614G, P681R), His,Avitag™

Specificité: Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
SARS-CoV-2 Spike S1 (T19R, G142D, EF156-157del, R158G, L452R, T478K, D614G, P681R)

Attributs du produit: Biotinylated SARS-CoV-2 Spike S1, His,Avitag is expressed from human 293 cells (HEK293). It contains AA Val 16 - Arg 685 (Accession # QHD43416.1). The mutations (T19R, G142D, EF156-157del, R158G, L452R, T478K, D614G, P681R) were identified in the SARS-CoV-2 variants which emerged in India (known as B.1.617.2).

Pureté: >95 % as determined by SDS-PAGE.

niveau d'endotoxine: Less than 1.0 EU per μg by the LAL method.

Information d'application

Commentaires:

Ready-to-use Avitag™ biotinylated protein:
The product is exclusively produced using the Avitag™ technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: PBS, pH 7.4

Stock: -20 °C

Référence

Erickson, Logsdon, Rhea, Hansen, Holden, Banks, Smith, German, Farr, Morley, Weaver, Hirsch, Kovac, Kontsekova, Baumann, Omer, Raber: "Blood-brain barrier penetration of non-replicating SARS-CoV-2 and S1 variants of concern induce neuroinflammation which is accentuated in a mouse model of Alzheimer's disease." dans: Brain, behavior, and immunity, Vol. 109, pp. 251-268, (2023) (PubMed).

Détail du antigène

Antigène: SARS-CoV-2 Spike S1

Abstract: SARS-CoV-2 Spike S1 Produits

Synonymes: E2 Protein, Surface Glycoprotein Protein, S Protein

Classe de substances: Viral Protein

Sujet: It's been reported that SARS-CoV-2 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Poids moléculaire: 78.4 kDa

NCBI Accession: QHD43416