ROBO1 Protein (His-Avi Tag,Biotin)

N° du produit ABIN7199171

Détail du produit

Antigène: ROBO1 (Roundabout, Axon Guidance Receptor, Homolog 1 (ROBO1))

Type de proteíne: Recombinant

Origine: Humain

Source: HEK-293 Cells

Purification/Conjugué: Cette ROBO1 protéine est marqué à la His-Avi Tag,Biotin.

Fonction: Biotinylated Human ROBO1 Protein, His,Avitag™ (MALS verified)

Séquence: GIn 26 - Pro 897

Attributs du produit: Biotinylated Human ROBO1, His,Avitag (RB1-H82E5) is expressed from human 293 cells (HEK293). It contains AA GIn 26 - Pro 897 (Accession # Q9Y6N7-1).

Pureté: >95 % as determined by SDS-PAGE.

niveau d'endotoxine: Less than 1.0 EU per μg by the LAL method.

Classe de qualité: MALS verified

Information d'application

Indications d'application: This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 99.2 kDa. The protein migrates as kDa under reducing (R) condition due to glycosylation.

Commentaires:

Ready-to-use Avitag™ biotinylated protein:
The product is exclusively produced using the Avitag™ technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: PBS, pH 7.4

Stock: -20 °C

Stockage commentaire: -20°C

Détail du antigène

Antigène: ROBO1 (Roundabout, Axon Guidance Receptor, Homolog 1 (ROBO1))

Autre désignation: ROBO1 (ROBO1 Produits)

Synonymes: DUTT1 Protein, SAX3 Protein, AW494633 Protein, AW742721 Protein, Gm310 Protein, ROBO1 Protein, dutt1 Protein, roundabout Protein, sax3 Protein, MGC122264 Protein, roundabout-1 Protein, roundabout guidance receptor 1 Protein, roundabout, axon guidance receptor, homolog 1 (Drosophila) Protein, roundabout guidance receptor 1 L homeolog Protein, ROBO1 Protein, Robo1 Protein, robo1 Protein, robo1.L Protein

Sujet: Synonyms: ROBO1/DUTT1,
Description: ROBO1 is a member of the ROBO immunoglobulin superfamily of proteins, and it plays a crucial role in cell motility and migration during embryogenesis and organogenesis. In addition, evidence showed that ROBO1 might drive migration and invasion in malignant cells, such as glioma and breast cancer, which might play a role in cancer aggressiveness. In contrast, some studies suggested that ROBO1 pathways play a key role in tumors by acting as a tumor suppressor, especially in cell invasion.

Poids moléculaire: 99.2 kDa

NCBI Accession: NP_002932

Pathways: Positive Regulation of Endopeptidase Activity