SIGLEC7 Protein (Glu201Ala-Mutant, Gly120Ala-Mutant, Leu118Ala-Mutant) (Fc Tag)

N° du produit ABIN7125986

Détail du produit

Antigène: SIGLEC7 (Sialic Acid Binding Ig-Like Lectin 7 (SIGLEC7))

Type de proteíne: Recombinant

Attributs du protein: Glu201Ala-Mutant, Gly120Ala-Mutant, Leu118Ala-Mutant

Origine: Humain

Source: HEK-293 Cells

Purification/Conjugué: Cette SIGLEC7 protéine est marqué à la Fc Tag.

Fonction: Human Siglec-7 / CD328 Protein, Fc (L118A, G120A, E201A) Tag (MALS verified)

Séquence: Gln 19 - Leu 353

Attributs du produit: Human Siglec-7, Fc (L118A, G120A, E201A) Tag is expressed from human 293 cells (HEK293). It contains AA Gln 19 - Leu 353 (Accession # Q9Y286-1).

Pureté: >95 % as determined by SDS-PAGE.

niveau d'endotoxine: Less than 0.01 EU per μg by the LAL method.

Classe de qualité: MALS verified

Information d'application

Indications d'application: This protein carries a Fc (L118A, G120A, E201A) tag at the C-terminus. The protein has a calculated MW of 63.3 kDa. The protein migrates as 70-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. Mutations (L118A, G120A, E201A /EU number: L235A/G237A/E318A) in human immunoglobulin G1 (hIgG1) Fc strongly reduce binding of the Fc mutant to cell expressed FcγRs, resulting in an almost 4-fold reduction in ADCC compared to that of wild type human IgG1.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: 25 mM MES, 150 mM NaCl

Stock: -20 °C

Stockage commentaire: -20°C

Détail du antigène

Antigène: SIGLEC7 (Sialic Acid Binding Ig-Like Lectin 7 (SIGLEC7))

Autre désignation: Siglec-7 / CD328 (SIGLEC7 Produits)

Synonymes: SIGLEC7 Protein, AIRM1 Protein, CD328 Protein, CDw328 Protein, D-siglec Protein, QA79 Protein, SIGLEC-7 Protein, SIGLEC19P Protein, SIGLECP2 Protein, p75 Protein, p75/AIRM1 Protein, sialic acid-binding Ig-like lectin 7 Protein, sialic acid binding Ig like lectin 7 Protein, LOC468976 Protein, SIGLEC7 Protein

Sujet: Synonyms: CDw328,D-siglec,A79 membrane protein,p75,Adhesion inhibitory receptor molecule 1, AIRM-1,
Siglec-7 is a member of the human CD33-related Siglec receptor. The extracellular region of Siglec-7 is characterized by an N-terminal V-set Ig domain that can bind sialic acid and two C2-set Ig domains. The cytoplasmic tail of Siglec-7 has one immune-receptor tyrosine-based inhibitory motif (ITIM) and one ITIM-like motif. Siglec-7 is considered as a sialic acid-dependent immunoreceptor with inhibitory potential and expressed predominantly on human NK cells, monocytes and a small subset of CD8+ T cells.

Poids moléculaire: 63.3 kDa

NCBI Accession: NP_055200