Annexin A11 Protein (ANXA11) (full length) (rho-1D4 tag)
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- Antigène Voir toutes Annexin A11 (ANXA11) Protéines
- Annexin A11 (ANXA11)
- Type de proteíne
- Recombinant
- Attributs du protein
- full length
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Origine
- CHO cells
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Source
- Cellules d'insectes
- Purification/Conjugué
- Cette Annexin A11 protéine est marqué à la rho-1D4 tag.
- Application
- SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys), Functional Studies (Func)
- Séquence
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MAQVLRGTVT AFPGFDSRAD AETLRKAMKG LGTDEESILT LLTSRSNAQR QEIAEEFKTL FGKDLLDDLK SELTGKFEKL IVALMKPSRL YDAYELKHAL KGAGTNEKVL TEIIASRTPE ELRVIKQVYE EEYGSSLEDD VVGDTSGYYQ RMLVVLLQAN RDPDTAIDDA QVELDAQALF QAGELKWGTD EEKFITIFGT RSVSHLRRVF DKYMTISGFQ IEETIDRETS GNLEQLLLAV VKSIRSIPAY LAETLYYAMK GAGTDDHTLI RVVVSRSEID LYNIRKEFRK NFATSLYSMI KGDTSGDYKK TLLLLCGGED D
Sequence without tag. The location of the tag depends on protein. You may also submit your preference when ordering. - Attributs du produit
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- Made in Germany - from design to production - by highly experienced protein experts.
- CHO Annexin Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a custom-made protein and will be made for the first time for your order. This protein will be produced on the basis of on a Custom Service Project. We will make sure that every step in the production is successful from the design of the expression plasmid to the expression and purification of the final protein. Our experts in the lab will ensure that you receive a correctly folded protein.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer. The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Three step purification of proteins expressed in baculovirus infected SF9 insect cells:
- Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
- The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Pureté
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Stérilité
- 0.22 μm filtered
- niveau d'endotoxine
- Endotoxins have not been removed. Please contact us if you require an endotoxin-free version of this product.
- Classe de qualité
- Crystallography grade
- Biological Activity Comment
- Protein has not been tested for activity yet.
- Top Product
- Discover our top product ANXA11 Protéine
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- Indications d'application
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 150 mM NaCL, 20 mM NaH2PO4 pH 7.4, 10 % glycerol. Note: Isoelectric point of protein taken into account regarding pH .
- Conseil sur la manipulation
- Avoid repeated freeze-thaw cycles.
- Stock
- -80 °C
- Stockage commentaire
- Store at -80°C.
- Date de péremption
- Unlimited (if stored properly)
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- Antigène
- Annexin A11 (ANXA11)
- Autre désignation
- Annexin (ANXA11 Produits)
- Synonymes
- anxa11 Protein, MGC89007 Protein, ANXA11 Protein, anx11 Protein, LOC100226075 Protein, cap50 Protein, ANX11 Protein, CAP50 Protein, A830099O17Rik Protein, Anx11 Protein, annexin A11 Protein, Annexin A11 Protein, annexin A11 S homeolog Protein, anxa11 Protein, ANXA11 Protein, anx11 Protein, anxa11.S Protein, Anxa11 Protein
- Sujet
- This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade. {ECO:0000256|ARBA:ARBA00002758}.
- UniProt
- G3I5A4
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