ADAM12 Protein (His tag)

N° du produit ABIN7317728

Détail du produit

Antigène: ADAM12 (ADAM Metallopeptidase Domain 12 (ADAM12))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: HEK-293 Cells

Purification/Conjugué: Cette ADAM12 protéine est marqué à la His tag.

Fonction: Recombinant Human ADAM12 Protein (His Tag)(Active)

Séquence: Met 1-Ser 513

Attributs du produit: A DNA sequence encoding the human ADAM12 isoform 1 (NP_003465.3) extracellular domain (Met 1-Ser 513) was fused with a polyhistidine tag at the C-terminus.

Pureté: > 98 % as determined by reducing SDS-PAGE.

niveau d'endotoxine: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: Measured by its binding ability in a functional ELISA.Immobilized human ADAM12-His at 10 μg/ml (100 μl/well) can bind biotinylated mouse FLRG-His with a linear range of 0.31-1.25 μg/ml.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Détail du antigène

Antigène: ADAM12 (ADAM Metallopeptidase Domain 12 (ADAM12))

Autre désignation: ADAM12 (ADAM12 Produits)

Synonymes: ADAM12 Protein, adam12 Protein, Mltna Protein, mKIAA4001 Protein, MCMP Protein, MCMPMltna Protein, MLTN Protein, MLTNA Protein, ADAM metallopeptidase domain 12 Protein, a disintegrin and metallopeptidase domain 12 (meltrin alpha) Protein, ADAM12 Protein, adam12 Protein, Adam12 Protein

Sujet:

Background: The ADAMs (a disintegrin and metalloprotease) comprise a family of multidomain proteins with metalloprotease, cell adhesion, and signaling activities. Human ADAM12, which is implicated in diseases such as cancer, is expressed in two splice forms, the transmembrane ADAM12-L and the shorter and soluble ADAM12-S. ADAM12, also known as and Meltrin alpha, is a member of the ADAM protein family, which contains one disintegrin domain, one EGF-like domain and one peptidase M12B domain. ADAM12 is synthesized as a zymogen with the prodomain keeping the metalloprotease inactive through a cysteine-switch mechanism. Maturation and activation of the protease involves the cleavage of the prodomain in the trans-Golgi or possibly at the cell surface by a furin-peptidase. It is a membrane-anchored metalloprotease, which has been implicated in activation-inactivation of growth factors that play an important role in wound healing, including heparin-binding epidermal growth factor (EGF)-like growth factor (HB-EGF) and IGF binding proteins. ADAM12 may also regulate cell-cell and cell-extracellular matrix contacts through interactions with cell surface receptors - integrins and syndecans - potentially influencing the actin cytoskeleton. Moreover, ADAM12 interacts with several cytoplasmic signaling and adaptor molecules through its intracellular domain, thereby directly transmitting signals to or from the cell interior. These ADAM12-mediated cellular effects appear to be critical events in both biological and pathological processes. In addition to protease activity, ADAM12 possesses cell binding and cell signaling properties. In many studies, ADAM12 overexpression has been correlated with disease, and ADAM12 has been shown to promote tumor growth and progression in cancer. On the other hand, protective effects of ADAM12 in disease have also been reported.

Synonym: ADAM12-OT1,CAR10,MCMP,MCMPMltna,MLTN,MLTNA

Poids moléculaire: 55.2 kDa

NCBI Accession: NP_003465

Pathways: EGFR Signaling Pathway