BCAM Protein (His tag)

N° du produit ABIN7321184

Détail du produit

Antigène: BCAM (Basal Cell Adhesion Molecule (Lutheran Blood Group) (BCAM))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Rat

Source: HEK-293 Cells

Purification/Conjugué: Cette BCAM protéine est marqué à la His tag.

Fonction: Recombinant Rat BCAM Protein (His Tag)(Active)

Séquence: Met1-Ala543

Attributs du produit: A DNA sequence encoding the rat BCAM (Q9ESS6) (Met1-Ala543) was expressed, fused with a polyhistidine tag at the C-terminus.

Pureté: > 90 % as determined by SDS-PAGE

niveau d'endotoxine: < 1.0 EU per μg of the protein as determined by the LAL method

Biological Activity Comment: Measured by the ability of the immobilized protein to support the adhesion of HOS human osteogenic sarcoma cells.When cells are added to coated plates(10 μg/mL,100μL/well), approximately 20-50% will adhere after 1 hour at 37°C.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Détail du antigène

Antigène: BCAM (Basal Cell Adhesion Molecule (Lutheran Blood Group) (BCAM))

Autre désignation: BCAM (BCAM Produits)

Synonymes: cd239 Protein, msk19 Protein, MGC81012 Protein, LU Protein, prr2 Protein, zgc:154034 Protein, 1200005K12Rik Protein, B-CAM Protein, Gplu Protein, Lu Protein, AU Protein, CD239 Protein, MSK19 Protein, basal cell adhesion molecule (Lutheran blood group) Protein, basal cell adhesion molecule (Lutheran blood group) L homeolog Protein, basal cell adhesion molecule Protein, bcam Protein, bcam.L Protein, BCAM Protein, Bcam Protein

Sujet:

Background: The Lutheran (Lu) blood group and basal cell adhesion molecule (BCAM) antigens are both carried by 2 glycoprotein isoforms of the immunoglobulin superfamily representing receptors for the laminin alpha(5) chain. It is a transmembrane receptor with five immunoglobulin-like domains in its extracellular region, and is therefore classified as a member of the immunoglobulin (Ig) gene family. In addition to red blood cells, Lu/BCAM proteins are expressed in endothelial cells of vascular capillaries and in epithelial cells of several tissues. BCAM/LU has a wide tissue distribution with a predominant expression in the basal layer of the epithelium and the endothelium of blood vessel walls. As designated as CD239 recently, BCAM and LU share a significant sequence similarity with the CD146 (MUC18) and CD166, and themselves are adhesion molecules that bind laminin with high affinity. Laminins are found in all basement membranes and are involved in cell differentiation, adhesion, migration, and proliferation. BCAM is upregulated following malignant transformation of some cell types in vivo and in vitro, thus being a candidate molecule involved in tumor progression. In addition, BCAM interacts with integrin in sickle red cells, and thus may potentially play a role in vaso-occlusive episodes.

Synonym: BCAM

Poids moléculaire: 58.2 kDa

UniProt: Q9ESS6