BLMH Protein (His tag)

N° du produit ABIN7194396

Détail du produit

Antigène: BLMH (Bleomycin Hydrolase (BLMH))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette BLMH protéine est marqué à la His tag.

Fonction: Recombinant Human BLMH/BLM Hydrolase Protein (His Tag)(Active)

Séquence: Ser 2-Glu 455

Attributs du produit: A DNA sequence encoding the human BLMH (NP_000377.1) (Ser 2-Glu 455) was expressed, with a polyhistide tag at the N-terminus.

Pureté: > 88 % as determined by reducing SDS-PAGE.

Biological Activity Comment: Measured by its ability to hydrolyze Met-AMC. The specific activity is >500 pmoles/min/μg.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Détail du antigène

Antigène: BLMH (Bleomycin Hydrolase (BLMH))

Autre désignation: BLMH/BLM Hydrolase (BLMH Produits)

Synonymes: AI035728 Protein, Bh Protein, Bmh Protein, wu:fb13c01 Protein, zgc:66261 Protein, bh Protein, bmh Protein, BH Protein, BMH Protein, bleomycin hydrolase Protein, Bleomycin hydrolase Protein, bleomycin hydrolase S homeolog Protein, BLMH Protein, pepC Protein, LOC5569945 Protein, Apre_0140 Protein, Apar_0899 Protein, Smon_1289 Protein, Kfla_1242 Protein, Snas_3912 Protein, BLJ_1941 Protein, Olsu_1085 Protein, PGTDC60_0106 Protein, Blmh Protein, blmh Protein, blmh.S Protein

Sujet:

Background: The papain superfamily member bleomycin hydrolase (BLMH) is a cytoplasmic cysteine peptidase that is highly conserved through evolution. The only known activity of the enzyme is metabolic inactivation of the glycopeptide bleomycin (BLM), an essential component of combination chemotherapy regimens for cancer. The papain superfamily member bleomycin hydrolase (BLMH) is a neutral cysteine protease with structural similarity to a 20S proteasome. Bleomycin (BLM), a clinically used glycopeptide anticancer agent. BLMH is an essential protectant against BLM-induced death and has an important role in neonatal survival and in maintaining epidermal integrity. Sequencing revealed several putative sites phosphorylated by different types of protein kinases, but no signal sequence, transmembrane domain, N-linked glycosylation site or DNA-binding motif.

Synonym: BH,BMH

Poids moléculaire: 53.4 kDa

NCBI Accession: NP_000377