CPA1 Protein (His tag)

N° du produit ABIN7194598

Détail du produit

Antigène: CPA1 (Carboxypeptidase A1 (Pancreatic) (CPA1))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Souris

Source: HEK-293 Cells

Purification/Conjugué: Cette CPA1 protéine est marqué à la His tag.

Fonction: Recombinant Mouse Carboxypeptidase A1/CPA1 Protein (His Tag)(Active)

Séquence: Met 1-Tyr 419

Attributs du produit: A DNA sequence encoding the mouse CPA1 (NP_079626.2) (Met 1-Tyr 419) precusor was expressed with a C-terminal polyhistidine tag.

Pureté: > 96 % as determined by SDS-PAGE

niveau d'endotoxine: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measured by its ability to cleave the colorimetric peptide substrate Ac-Phe-Thiaphe-OH in the presence of 5,5'Dithiobis (2-nitrobenzoic acid) (DTNB). The specific activity is >6,000 pmoles/min/μg.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 20 mM Tris, 150 mM NaCl, pH 7.5

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Détail du antigène

Antigène: CPA1 (Carboxypeptidase A1 (Pancreatic) (CPA1))

Autre désignation: Carboxypeptidase A1/CPA1 (CPA1 Produits)

Synonymes: cpa1 Protein, cbpa1 Protein, CPA Protein, CPA1 Protein, 0910001L12Rik Protein, Cpa Protein, carboxypeptidase A1 Protein, pancreatic carboxypeptidase A1 Protein, Carboxypeptidase A1 Protein, carboxypeptidase A1-like Protein, carboxypeptidase a1 Protein, carboxypeptidase A1, pancreatic Protein, CpipJ_CPIJ007169 Protein, CpipJ_CPIJ010802 Protein, CpipJ_CPIJ010805 Protein, MCYG_06789 Protein, MGYG_03840 Protein, cpa1 Protein, cbpa1 Protein, LOC100224866 Protein, CPA1 Protein, Cpa1 Protein

Sujet:

Background: Carboxypeptidase A1 (CPA1)is secreted as a pancreatic procarboxypeptidase, and cleaves the C-terminal amide or ester bond of peptides that have a free C-terminal carboxyl group, with the preference of  residues with aromatic or branched aliphatic side chains. CPA1 comprises a signal peptide, a pro region and a mature chain, and can be activated after cleavage of the pro peptide. In contrast to procarboxypeptidase B which was always secreted by the pancreas as a monomer, procarboxypeptidase A occurs as a monomer and/or associated to one or two functionally different proteins, such as zymogen E, and is involved in zymogen inhibition. Three different forms of human pancreatic procarboxypeptidase A have been isolated.

Synonym: 0910001L12Rik;Cpa

Poids moléculaire: 47 kDa

NCBI Accession: NP_079626