CD40 Ligand Protein (CD40LG) (AA 113-261) (His tag)

N° du produit ABIN7319660

Protéine Recombinant CD40 Ligand exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour CD40 Ligand Protein (CD40LG) (AA 113-261) (His tag) (ABIN7319660)

Antigène: CD40 Ligand (CD40LG)

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: Escherichia coli (E. coli)

Application: Cell Culture (CC)

Pureté: > 95 % as determined by reducing SDS-PAGE.

Détail du produit

Attributs du protein: AA 113-261

Purification/Conjugué: Cette CD40 Ligand protéine est marqué à la His tag.

Fonction: Recombinant Human CD40L/TNFSF5 Protein (E.coli)

Séquence: Met113-Leu261

Attributs du produit: Recombinant Human CD40L is produced by our E.coli expression system and the target gene encoding Met113-Leu261 is expressed.

Stérilité: 0.2 μm filtered

niveau d'endotoxine: < 0.1 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measure by its ability to induce IL-8 secretion in human PBMCs. The ED₅₀ for this effect is <5 ng/mL.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 8.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Date de péremption: 12 months

Détail du antigène

Antigène: CD40 Ligand (CD40LG)

Autre désignation: CD40L/TNFSF5

Sujet: CD154,CD40 Ligand,CD40-L,CD40L,CD40LG,HIGM1,IGM,IMD3,T-BAM,T-Cell Antigen Gp39,TNF-Related Activation Protein,TNFSF5,TRAP,Tumor Necrosis Factor Ligand Superfamily Member 5,hCD40L,CD40 Ligand (CD40LG) is a type II transmembrane glycoprotein that belongs to the TNF superfamily. Like other TNF superfamily members, CD40LG exists as a trimer in membrane bound and soluble form, both of which are bioactive. CD40LG is a ligand for CD40, its ligation also initiates signal transduction in CD40LG expressing cells. CD40LG is a differentiation antigen that is expressed on the surface of T-cells. It stimulates B-cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines. CD40LG has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes. It also co-stimulates proliferation of activated T-cells and this is accompanied by the production of IFN-gamma, TNF-alpha, and IL2.

Poids moléculaire:

Calculated MW: 17.0 kDa

Observed MW: 16 kDa

ID gène: 959

UniProt: P29965

Pathways: Signalisation NF-kappaB, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints