CSTB Protein (His tag)

N° du produit ABIN7317867

Détail du produit

Antigène: CSTB (Cystatin B (Stefin B) (CSTB))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette CSTB protéine est marqué à la His tag.

Fonction: Recombinant Human Cystatin B/CSTB Protein (His Tag)(Active)

Séquence: Met 2-Phe 98

Attributs du produit: A DNA sequence encoding the human CSTB (P04080) (Met 2-Phe 98) was expressed, with a polyhistide tag at the N-terminus.

Pureté: > 95 % as determined by reducing SDS-PAGE.

Biological Activity Comment: Measured by its ability to inhibit papain cleavage of a fluorogenic peptide substrate Z-FR-AMC (R&D Systems, Catalog # ES009). The IC50 value is < 15 nM.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 50 mM Tris, 50 mM NaCl, pH 8.0

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Détail du antigène

Antigène: CSTB (Cystatin B (Stefin B) (CSTB))

Autre désignation: Cystatin B/CSTB (CSTB Produits)

Synonymes: CST6 Protein, EPM1 Protein, EPM1A Protein, PME Protein, STFB Protein, ULD Protein, CSTB Protein, Cyb Protein, Epm1 Protein, Stfb Protein, MGC189005 Protein, pme Protein, cst6 Protein, epm1 Protein, stfb Protein, AA960480 Protein, ARABIDOPSIS THALIANA PHYTOCYSTATIN 6 Protein, ATCYS6 Protein, ATCYSB Protein, cystatin B Protein, LOC100008600 Protein, LOC100136235 Protein, CPI1 Protein, cystatin B Protein, cystatin B (stefin B) Protein, cystatin 14a, tandem duplicate 2 Protein, cystatin-B Protein, CSTB Protein, Cstb Protein, cstb Protein, cst14a.2 Protein, CYSB Protein, cpi1 Protein, LOC101123265 Protein

Sujet:

Background: Cystatin-B, also known as CPI-B, Liver thiol proteinase inhibitor, Stefin-B, CSTB and CST6, is a cytoplasm and nucleus protein which belongs to the cystatin family. Cystatin-B / CSTB is an intracellular thiol proteinase inhibitor. Tightly binding reversible inhibitor of cathepsins L, H and B. Cystatin-B / CSTB is able to form a dimer stabilized by noncovalent forces, inhibiting papain and cathepsins l, h and b. Cystatin-B / CSTB is also thought to play a role in protecting against the proteases leaking from lysosomes. Defects in Cystatin-B / CSTB are the cause of progressive myoclonic epilepsy type 1 (EPM1) which is an autosomal recessive disorder characterized by severe, stimulus-sensitive myoclonus and tonic-clonic seizures. The cystatins are a family of cysteine protease inhibitors with homology to chicken cystatin. Cystatins are physiological inhibitors of cysteine proteinases which are widely distributed in human tissues and fluids. Cystatins typically comprise about 115 amino acids, are largely acidic, contain four conserved cysteine residues known to form two disulfide bonds. Cystatins may be glycosylated and / or phosphorylated, with similarity to fetuins, kininogens, stefins, histidine-rich glycoproteins and cystatin-related proteins. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired inhibitory activity. Cystatins mainly inhibit peptidases belonging to peptidase families C1 (papain family) and C13 (legumain family).

Synonym: CST6;EPM1;EPM1A;PME;STFB;ULD

Poids moléculaire: 12.5 kDa

UniProt: P04080

Pathways: Response to Water Deprivation