EDF1 Protein (His tag)

N° du produit ABIN7318429

Détail du produit

Antigène: EDF1 (Endothelial Differentiation Related Factor 1 (EDF1))

Type de proteíne: Recombinant

Origine: Humain

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette EDF1 protéine est marqué à la His tag.

Fonction: Recombinant Human EDF1/MBF1 Protein (His Tag)

Séquence: Ala2-Lys148

Attributs du produit: Recombinant Human Endothelial Differentiation-Related Factor 1 is produced by our E.coli expression system and the target gene encoding Ala2-Lys148 is expressed with a 6His tag at the C-terminus.

Pureté: > 95 % as determined by reducing SDS-PAGE.

niveau d'endotoxine: < 1.0 EU per μg as determined by the LAL method.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM TrisHCl, 150 mM NaCl, pH 8.0.

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Détail du antigène

Antigène: EDF1 (Endothelial Differentiation Related Factor 1 (EDF1))

Autre désignation: EDF1/MBF1 (EDF1 Produits)

Synonymes: edf-1 Protein, mbf1 Protein, EDF-1 Protein, MBF1 Protein, 0610008L11Rik Protein, AA409425 Protein, CAP-19 Protein, zgc:73192 Protein, zgc:86829 Protein, endothelial differentiation related factor 1 S homeolog Protein, endothelial differentiation related factor 1 Protein, endothelial differentiation-related factor 1 Protein, Mbf1p Protein, edf1.S Protein, EDF1 Protein, Edf1 Protein, edf1 Protein, MBF1 Protein

Sujet:

Background: Endothelial Differentiation-Related Factor 1 (EDF1) is a 148 amino acid transcriptional coactivator that contains 1 HTH cro/C1-type DNA-binding domain. It has been postulated that the protein functions as a bridging molecule that interconnects regulatory proteins and the basal transcriptional machinery, thereby modulating the transcription of genes involved in endothelial differentiation. When endothelial cells are induced to differentiate in vitro, EDF1 is downregulated, leading to inhibition of cell growth and cell polarization. EDF1 binds calmodulin thorough its IQ domain and regulates nitric oxide synthase activity through calmodulin sequestration in the cytoplasm. Though ubiquitously expressed, EDF1 is most abundant in adult liver, heart, adipose tissues, intestine and pancreas. In fetal tissues, EDF1 is most abundant in kidney. There are two isoforms of EDF1 that are produced as a result of alternative splicing events.

Synonym: Endothelial Differentiation-Related Factor 1, EDF-1, Multiprotein-Bridging Factor 1, MBF1, EDF1

Poids moléculaire: 17.4 kDa

UniProt: O60869