FCGR2B Protein (His tag)

N° du produit ABIN7195694

Détail du produit

Antigène: FCGR2B (Fc Fragment of IgG, Low Affinity IIb, Receptor (CD32) (FCGR2B))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Rat

Source: HEK-293 Cells

Purification/Conjugué: Cette FCGR2B protéine est marqué à la His tag.

Fonction: Recombinant Rat CD32b/FCGR2B Protein (His Tag)(Active)

Séquence: Met 1-Pro 212

Attributs du produit: A DNA sequence encoding the rat FCGR2B (Q63203-1) extracellular domain (Met 1-Pro 212) was expressed, fused with a polyhistidine tag at the C-terminus.

Pureté: > 97 % as determined by SDS-PAGE

niveau d'endotoxine: < 1.0 EU per μg of the protein as determined by the LAL method

Biological Activity Comment: Measured by its binding ability in a functional ELISA.Immobilized human rat FCGR2B-His at 10 μg/ml (100 μl/well) can bind biotinylated human IgG1, The EC50 of biotinylated human IgG1 is 0.3-0.8 μg/ml.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Détail du antigène

Antigène: FCGR2B (Fc Fragment of IgG, Low Affinity IIb, Receptor (CD32) (FCGR2B))

Autre désignation: CD32b/FCGR2B (FCGR2B Produits)

Synonymes: CD32 Protein, CD32B Protein, FCG2 Protein, FCGR2 Protein, IGFR2 Protein, FCGR2B Protein, AI528646 Protein, F630109E10Rik Protein, Fc[g]RII Protein, FcgRII Protein, Fcgr2 Protein, Fcgr2a Protein, Fcr-2 Protein, Fcr-3 Protein, Ly-17 Protein, Ly-m20 Protein, LyM-1 Protein, Lym-1 Protein, FCRII Protein, FcRII Protein, Fc fragment of IgG receptor IIb Protein, Fc fragment of IgG, low affinity IIb, receptor (CD32) Protein, Fc receptor, IgG, low affinity IIb Protein, low affinity immunoglobulin gamma Fc region receptor II Protein, FCGR2B Protein, Fcgr2b Protein, LOC100856270 Protein

Sujet:

Background: FcγRIIB is a low affinity receptor that recognizes the Fc portion of IgG. The human CD32 group consists of FcγRIIA, FcγRIIB, and FcγRIIC proteins that share 94-99 % sequence identity in their extracellular domains but differ substantially in their transmembrane and cytoplasmic domains. FcγRII protein is expressed on cells of both myeloid and lymphoid lineages as well as on cells of non-hematopoietic origin. FcγRIIB has an intrinsic cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM) and delivers an inhibitory signal upon ligand binding. Ligation of FcγRIIB on B cells down-regulates antibody production and in some circumstances may promote apoptosis. Co-ligation of FcγRIIB on dendritic cells inhibits maturation and blocks cell activation. FcγRIIB may also be a target for monoclonal antibody therapy for malignancies. FcγRIIB plays an important negative-regulating role through modulating the signals from activation receptors.

Synonym: FCRII,Fcgr2

Poids moléculaire: 21.9 kDa

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, BCR Signaling