FGF9 Protein (His tag)

N° du produit ABIN7320527

Cette protéine Recombinant FGF9 est exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour FGF9 Protein (His tag) (ABIN7320527)

Antigène: FGF9 (FGF-9) (Fibroblast Growth Factor 9 (FGF-9))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Souris

Source: Escherichia coli (E. coli)

Pureté: > 95 % as determined by SDS-PAGE

Détail du produit

Purification/Conjugué: Cette FGF9 protéine est marqué à la His tag.

Fonction: Recombinant Mouse FGF-9/FGF9 Protein (His Tag)(Active)

Séquence: Met1-Ser208

Attributs du produit: Recombinant Mouse Fibroblast Growth Factor 9 is produced by our E.coli expression system and the target gene encoding Met1-Ser208 is expressed with a 6His tag at the C-terminus.

niveau d'endotoxine: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 4.14 ng/ml.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Frozen, Liquid

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM Tris,150 mM NaCl,5 % Trehalose,1 mM EDTA,20 %glycerol,1 mM DTT, pH 8.5 .

Stock: -20 °C

Stockage commentaire: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Détail du antigène

Antigène: FGF9 (FGF-9) (Fibroblast Growth Factor 9 (FGF-9))

Autre désignation: FGF-9/FGF9

Sujet:

Background: Fibroblast growth factor-9 (FGF-9) is an approximately 26 kDa secreted glycoprotein of the FGF family. Secreted mouse FGF-9 lacks the N-terminal 1-3 aa and shares >98 % sequence identity with rat, human, equine, porcine and bovine FGF-9. FGF-9 plays an important role in the regulation of embryonic development, cell proliferation, cell differentiation and cell migration. In the mouse embryo the location and timing of FGF-9 expression affects development of the skeleton, cerebellum, lungs, heart, vasculature, digestive tract, and testes .It may have a role in glial cell growth and differentiation during development, gliosis during repair and regeneration of brain tissue after damage, differentiation and survival of neuronal cells, and growth stimulation of glial tumors. Deletion of mouse FGF-9 is lethal at birth due to lung hypoplasia, and causes rhizomelia, or shortening of the proximal skeleton. An unusual constitutive dimerization of FGF 9 buries receptor interaction sites which lowers its activity, and increases heparin affinity which inhibits diffusion. A spontaneous mouse mutant, Eks, interferes with dimerization, resulting monomeric, diffusible FGF-9 that causes elbow and knee synostoses (joint fusions) due to FGF-9 misexpression in developing joints.

Synonym: Fibroblast growth factor 9,FGF-9,Glia-activating factor,GAF,heparin-binding growth factor-9,HBGF-9,Fgf9,Fgf-9

Poids moléculaire: 24.4 kDa

UniProt: P54130