IL-6 Protein (AA 25-211)

N° du produit ABIN7321248

Cette protéine Recombinant IL-6 est produite dans Escherichia coli (E. coli).

Aperçu rapide pour IL-6 Protein (AA 25-211) (ABIN7321248)

Antigène: IL-6 (IL6) (Interleukin 6 (IL6))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Rat

Source: Escherichia coli (E. coli)

Application: Cell Culture (CC)

Pureté: > 90 % as determined by reducing SDS-PAGE.

Détail du produit

Attributs du protein: AA 25-211

Fonction: Recombinant Rat IL-6/Interleukin-6 Protein

Séquence: Phe25-Thr211

Attributs du produit: A DNA sequence encoding the rat IL6 (Phe25-Thr211) was expressed and purified with an initial Met.

Stérilité: 0.2 μm filtered

Biological Activity Comment: Measured in a cell proliferation assay using T1165 mouse plasmacytoma cells. The ED₅₀ for this effect is typically 1-6 ng/ml.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: Lyophilized from sterile 40 % acetonitrile, 0.1 % TFA
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Date de péremption: 12 months

Détail du antigène

Antigène: IL-6 (IL6) (Interleukin 6 (IL6))

Autre désignation: IL-6/Interleukin-6

Sujet: IL6,Interleukin-6 (IL-6) is a multifunctional α-helical cytokine that regulates cell growth and differentiation of various tissues, which is known particularly for its role in the immune response and acute phase reactions. IL-6 protein is secreted by a variety of cell types including T cells and macrophages as phosphorylated and variably glycosylated molecule. It exerts actions through the its heterodimeric receptor composed of IL-6R that lacks the tyrosine/kinase domain and binds IL-6 with low affinity, and ubiquitously expressed glycoprotein 130 (gp130) that binds the IL-6. IL-6R complex with high affinity and thus transduces signals. IL-6 is also involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined an essential role in directing transition from innate to acquired immunity.

Poids moléculaire:

Calculated MW: 21.9 kDa

Observed MW: 22 kDa

NCBI Accession: NP_036721

UniProt: A6KJN3

Pathways: Signalisation TLR, Hormone Transport, Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Autophagy, Cell RedoxHomeostasis, Cancer Immune Checkpoints, Inflammasome