Intestinal Alkaline Phosphatase Protein (ALPI) (His tag)
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- Antigène Voir toutes Intestinal Alkaline Phosphatase (ALPI) Protéines
- Intestinal Alkaline Phosphatase (ALPI)
- Type de proteíne
- Recombinant
- Activité biologique
- Active
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Origine
- Humain
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Source
- HEK-293 Cells
- Purification/Conjugué
- Cette Intestinal Alkaline Phosphatase protéine est marqué à la His tag.
- Fonction
- Recombinant Human Alkaline Phosphatase/ALPI Protein (His Tag)(Active)
- Séquence
- Met 1-Asp 503
- Attributs du produit
- A DNA sequence encoding the mature form of human ALPI (P09923) (Met 1-Asp 503),without the pro peptide, was fused with a polyhistidine tag at the C-terminus.
- Pureté
- > 95 % as determined by reducing SDS-PAGE.
- niveau d'endotoxine
- < 1.0 EU per μg of the protein as determined by the LAL method.
- Biological Activity Comment
- Measured by its ability to cleave a fluorogenic substrate, 4-Methylumbelliferyl phosphate (4-MUP).The specific activity is > 1,000 pmoles/min/μg.
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Intestinal Alkaline Phosphatase (ALPI) (AA 74-162) protein (GST tag)
ALPI Origine: Humain Hôte: Wheat germ Recombinant WB, ELISA, AA, AP
Intestinal Alkaline Phosphatase (ALPI) (AA 1-557) protein (Strep Tag)Crystallography grade ALPI Origine: Souris Hôte: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Intestinal Alkaline Phosphatase (ALPI) (AA 20-503) protein (His tag)Crystallography grade ALPI Origine: Humain Hôte: Cellules d'insectes Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Intestinal Alkaline Phosphatase (ALPI) (AA 20-528) protein (His tag)Crystallography grade ALPI Origine: Souris Hôte: Cellules d'insectes Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Intestinal Alkaline Phosphatase (ALPI) protein (Myc-DYKDDDDK Tag)ALPI Origine: Humain Hôte: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Please refer to the printed manual for detailed information.
- Buffer
- Lyophilized from sterile PBS, pH 7.4
- Stock
- 4 °C,-20 °C,-80 °C
- Stockage commentaire
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- Antigène
- Intestinal Alkaline Phosphatase (ALPI)
- Autre désignation
- Alkaline Phosphatase/ALPI (ALPI Produits)
- Synonymes
- IAP Protein, Alp1 Protein, S51097 Protein, 2010001C14Rik Protein, Akp6 Protein, gIAP Protein, alpi Protein, im:7149588 Protein, si:ch211-262h13.1 Protein, wu:fa95f04 Protein, zgc:110794 Protein, ALPI Protein, Alpi Protein, alkaline phosphatase, intestinal Protein, alkaline phosphatase, intestinal, tandem duplicate 1 Protein, intestinal-type alkaline phosphatase Protein, ALPI Protein, Alpi Protein, alpi.1 Protein, LOC100724954 Protein
- Sujet
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Background: ALPI encodes for intestinal phosphatase alkaline, a brush border metalloenzyme that hydrolyses phosphate from the lipid A moiety of lipopolysaccharides and thereby drastically reduces Toll-like receptor 4 agonist activity. ALPI mutations impaired either stability or catalytic activity of ALPI and rendered it unable to detoxify lipopolysaccharide-dependent signalling. ALPI mutations should be included in screening for monogenic causes of inflammatory bowel diseases and lay the groundwork for ALPI-based treatments in intestinal inflammatory disorders.
Synonym: IAP
- Poids moléculaire
- 54 kDa
- UniProt
- P09923
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