LTC4S Protein (His tag)

N° du produit ABIN7317520

Détail du produit

Antigène: LTC4S (Leukotriene C4 Synthase (LTC4S))

Type de proteíne: Recombinant

Origine: Humain

Source: Baculovirus infected Insect Cells

Purification/Conjugué: Cette LTC4S protéine est marqué à la His tag.

Fonction: Recombinant Human LTC4S/LTC4 synthase Protein (His Tag)

Séquence: Met 1-Ala 150

Attributs du produit: A DNA sequence encoding the human LTC4S (NP_665874.1) (Met 1-Ala 150) was fused with a polyhistidine tag at the C-terminus.

Pureté: > 90 % as determined by reducing SDS-PAGE.

niveau d'endotoxine: < 1.0 EU per μg as determined by the LAL method.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 50 mM Hepes, 0.1 % Triton 0.5 % DOC, 10 % glycerol, pH 8.0

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Détail du antigène

Antigène: LTC4S (Leukotriene C4 Synthase (LTC4S))

Autre désignation: LTC4S/LTC4 synthase (LTC4S Produits)

Synonymes: LTC4S Protein, ltc4s Protein, si:dkey-33h4.2 Protein, leukotriene C4 synthase Protein, leukotriene C4 synthase-like Protein, leukotriene C4 synthase, gene 1 Protein, Ltc4s Protein, LTC4S Protein, LTC4SL Protein, ltc4s.1 Protein, ltc4s Protein

Sujet:

Background: Leukotriene C4 synthase, also known as LTC4 synthase, Leukotriene-C(4) synthase, and LTC4S, is a multi-pass membrane protein which belongs to the MAPEG family. LTC4S is detected in lung, platelets and the myelogenous leukemia cell line KG-1 (at protein level). LTC4S activity is present in eosinophils, basophils, mast cells, certain phagocytic mononuclear cells, endothelial cells, vascular smooth muscle cells and platelets. LTC4S is essential for the production of cysteinyl leukotrienes (Cys-LT), critical mediators in asthma. Mutagenic analysis of the conjugation function of human LTC4S has identified R51 and Y93 as critical for acid and base catalysis of LTA4 and reduced glutathione, respectively. A comparison across species for proteins that possess LTC4S activity reveals conservation of both of these residues, whereas R51 is absent in the FLAP molecules. Thus, within the glutathione S-transferase superfamily of genes, alignment of specific residues allows the separation of LTC4S family members from their most structurally similar counterparts, the FLAP molecules. Defects in LTC4S are the cause of leukotriene C4 synthase deficiency (LTC4 synthase deficiency). LTC4 synthase deficiency is a fatal neurometabolic developmental disorder. It is associated with muscular hypotonia, psychomotor retardation, failure to thrive, and microcephaly.

Synonym: LTC4S;MGC33147

Poids moléculaire: 17 kDa

NCBI Accession: NP_665874

Pathways: Cellular Response to Molecule of Bacterial Origin