MEP1A Protein (His tag)

N° du produit ABIN7196977

Détail du produit

Antigène: MEP1A (Meprin A, alpha (PABA Peptide Hydrolase) (MEP1A))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Souris

Source: HEK-293 Cells

Purification/Conjugué: Cette MEP1A protéine est marqué à la His tag.

Fonction: Recombinant Mouse MEP1A/PPHA Protein (His Tag)(Active)

Séquence: Met 1-Arg 615

Attributs du produit: A DNA sequence encoding the mouse MEP1A (NP_032611.2) (Met 1-Arg 615) was expressed, fused with a polyhistidine tag at the C-terminus.

Pureté: > 95 % as determined by SDS-PAGE

niveau d'endotoxine: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measured by its ability to cleave a fluorogenic peptide substrate, Mca-YVADAPK(Dnp)-OH, R&D Systems, Catalog # ES007.The specific activity is >400 pmoles/min/μg.(Activation description: The proenzyme needs to be activated by Trypsin for an activated form)

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Détail du antigène

Antigène: MEP1A (Meprin A, alpha (PABA Peptide Hydrolase) (MEP1A))

Autre désignation: MEP1A/PPHA (MEP1A Produits)

Synonymes: AI098089 Protein, AW107200 Protein, Mep-1 Protein, Mep-1a Protein, Mep1 Protein, PPHA Protein, meprin A subunit alpha Protein, meprin 1 alpha Protein, Mep1a Protein, MEP1A Protein

Sujet:

Background: Meprin A subunit alpha, also known as MEP1A, and Endopeptidase-2, is a single-pass type I  membrane protein which belongs to the peptidase M12A family. MEP1A contains one EGF-like domain, one MAM domain, and one MATH domain. Meprins are unique plasma membrane and secreted metalloproteinases that are highly regulated at the transcriptional and post-translational levels. Meprin alpha and beta subunits are abundantly expressed in kidney and intestinal epithelial cells, are secreted into the urinary tract and intestinal lumen, and are found in leukocytes and cancer cells under certain conditions. Meprins are capable of proteolytically degrading extracellular matrix proteins, proteolytically processing bioactive proteins, and play a role in inflammatory processes. Meprin A and B are highly regulated, secreted and cell-surface homo- and hetero-oligomeric enzymes. Meprins are abundantly expressed in kidney and intestine. The multidomain alpha and beta subunits have high sequence identity. They have very different substrate specificities, oligomerization potentials and are differentially regulated. Meprin A appears to be an important therapeutic target and urinary excretion appears to be a potential biomarker of acute kidney injury ( AKI ).

Synonym: AI098089;AW107200;Mep-1;Mep-1a;Mep1

Poids moléculaire: 69 kDa

NCBI Accession: NP_032611