B7-H6 Protein (His tag)
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- Antigène Voir toutes B7-H6 (NCR3LG1) Protéines
- B7-H6 (NCR3LG1) (Natural Killer Cell Cytotoxicity Receptor 3 Ligand 1 (NCR3LG1))
- Type de proteíne
- Recombinant
- Activité biologique
- Active
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Origine
- Humain
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Source
- HEK-293 Cells
- Purification/Conjugué
- Cette B7-H6 protéine est marqué à la His tag.
- Fonction
- Recombinant Human B7-H6 Protein (His Tag)(Active)
- Séquence
- Met 1-Ser262
- Attributs du produit
- A DNA sequence encoding the human NCR3LG1 (NP_001189368.1) (Met1-Ser262) was expressed with a polyhistidine tag at the C-terminus.
- Pureté
- > 95 % as determined by reducing SDS-PAGE.
- niveau d'endotoxine
- < 1.0 EU per μg of the protein as determined by the LAL method.
- Biological Activity Comment
- Measured by its binding ability in a functional ELISA. Immobilized human B7-H6 -His at 10μg/mL (100μL/well) can bind human NCR3-Fch, the EC50 of human NCR3-Fch is 6-200ng/mL.
- Top Product
- Discover our top product NCR3LG1 Protéine
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Please refer to the printed manual for detailed information.
- Buffer
- Lyophilized from sterile PBS, pH 7.4
- Stock
- 4 °C,-20 °C,-80 °C
- Stockage commentaire
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- Antigène
- B7-H6 (NCR3LG1) (Natural Killer Cell Cytotoxicity Receptor 3 Ligand 1 (NCR3LG1))
- Autre désignation
- B7-H6 (NCR3LG1 Produits)
- Synonymes
- B7-H6 Protein, B7H6 Protein, DKFZp686O24166 Protein, natural killer cell cytotoxicity receptor 3 ligand 1 Protein, NCR3LG1 Protein
- Sujet
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Background: Natural cytotoxicity triggering receptor 3 ligand 1(B7-H6) is a glycosylated member of the B7 family of immune costimulatory proteins. Mature human B7-H6 consists of a 238 amino acid (aa) extracellular domain (ECD) that contains one Ig-like V domain and one Ig-like C1 domain, a 21 aa transmembrane segment, and a 171 aa cytoplasmic domain that contains one ITIM, one SH2, and one SH3 motif. Both of the Ig-like domains carry N-linked glycosylation. The Ig-like V domain mediates 1:1 stoichiometric binding of B7-H6 to NKp30 expressed on NK cells. It does not show binding to NKp44, NKp46, or NKG2D. Ligation of NKp30 by B7-H6 induces NK cell activation and target cell cytolysis. B7-H6 is expressed on a wide range of hematopoietic, carcinoma, and melanoma tumor cells, which is consistent with the detection of NKp30 binding sites on many tumors.
Synonym: B7-H6,B7H6,DKFZp686O24166
- Poids moléculaire
- 28.1 kDa
- NCBI Accession
- NP_001189368
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