RSPO2 Protein (Fc Tag)

N° du produit ABIN7197624

Détail du produit

Antigène: RSPO2 (R-Spondin 2 (RSPO2))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: HEK-293 Cells

Purification/Conjugué: Cette RSPO2 protéine est marqué à la Fc Tag.

Fonction: Recombinant Human FTLS/RSPO2 Protein (186 Leu/Pro, Fc Tag)(Active)

Séquence: Met 1-Gly 205, 186 Leu/Pro

Attributs du produit: A DNA sequence encoding the human RSPO2 (Q6UXX9-1) N-teminal fragment (Met 1-Gly 205, 186 Leu/Pro) was fused with the Fc region of human IgG1 at the C-terminus.

Pureté: > 90 % as determined by reducing SDS-PAGE.

niveau d'endotoxine: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: Measured by its ability to induce activation of β-catenin response in a Topflash Luciferase assay using HEK293T human embryonic kidney cells.The ED50 for this effect is typically 10-60 ng/mL in the presence of 5 ng/mL recombinant mouse Wnt3a.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Détail du antigène

Antigène: RSPO2 (R-Spondin 2 (RSPO2))

Autre désignation: FTLS/RSPO2 (RSPO2 Produits)

Synonymes: RGD1562331 Protein, CRISTIN2 Protein, 2610028F08Rik Protein, AA673245 Protein, D430027K22 Protein, ftls Protein, R-spondin 2 Protein, R-spondin 2 L homeolog Protein, Rspo2 Protein, rspo2.L Protein, rspo2 Protein, RSPO2 Protein

Sujet:

Background: R-spondin-2, also known as RSPO2, synergizes with Wnt to activate beta-catenin. RSPO2 is secreted proteins that regulate beta-catenin signaling. Activator of the beta-catenin signaling cascade leads to TCF-dependent gene activation. Action both in the canonical Wnt / beta- catenin-dependent pathway, possibly via a direct interaction with Wnt proteins, and in a Wnt-independent beta catenin pathway through a receptor signaling pathway that may not use frizzled / LRP receptors. Probably also acts as a ligand for frizzled and LRP receptors. The encoding gene Rspo2 was identified as a novel common integration site for the mouse mammary tumor virus in viral induced mouse mammary tumors. Rspo2 and Rspo2 / Wnt1 tumors contained many spindle cells, consistent with an epithelial-mesenchymal transformation phenotype. When Rspo2 and Rspo2 / Wnt1 tumor cells were transferred into naive mice, they exhibited greater metastatic activity than cells derived from Wnt1 tumors.

Synonym: CRISTIN2

Poids moléculaire: 48.3 kDa