SPARC Protein (His tag)

N° du produit ABIN7318851

Détail du produit

Antigène: SPARC (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))

Type de proteíne: Recombinant

Origine: Humain

Source: Human Cells

Purification/Conjugué: Cette SPARC protéine est marqué à la His tag.

Fonction: Recombinant Human Osteonectin/SPARC Protein (His Tag)

Séquence: Ala18-Ile303

Attributs du produit: Recombinant Human Secreted Protein Acidic and Rich in Cysteine is produced by our Mammalian expression system and the target gene encoding Ala18-Ile303 is expressed with a 6His tag at the C-terminus.

Pureté: > 95 % as determined by reducing SDS-PAGE.

niveau d'endotoxine: < 1.0 EU per μg as determined by the LAL method.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2.

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Détail du antigène

Antigène: SPARC (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))

Autre désignation: Osteonectin/SPARC (SPARC Produits)

Synonymes: ON Protein, AA517111 Protein, BM-40 Protein, hm:zeh0062 Protein, SPARC Protein, sparc Protein, secreted protein acidic and cysteine rich Protein, secreted acidic cysteine rich glycoprotein Protein, secreted protein, acidic, cysteine-rich (osteonectin) Protein, secreted protein, acidic, cysteine-rich (osteonectin) L homeolog Protein, secreted protein, acidic, cysteine-rich (osteonectin) S homeolog Protein, SPARC Protein, Sparc Protein, sparc Protein, sparc.L Protein, sparc.S Protein

Sujet:

Background: Secreted Protein Acidic and Rich in Cysteine (SPARC) is a secreted, evolutionarily conserved collagen-binding glycoprotein and belongs to the SPARC family. SPARC has 286 amino acids and contains an EF-hand in C-termina domain, a follistatin-like domain with Kazal-like sequences. There are two calcium binding sites, one binds 5 - 8 Ca2+ with a low affinity and other on an EF-hand loop that binds a Ca2+ ion with a high affinity. It is highly expressed in tissues undergoing morphogenesis, remodeling and wound repair. SPARC regulate cell growth through interactions with the extracellular matrix (ECM) and cytokines. SPARC bind to numerous proteins of the ECM, affect ECM protein expression, influence cellular adhesion and migration, and modulate growth factor-induced cell proliferation and angiogenesis. SPARC also binds several types of collagen, albumin, thrombospondin, PDGF and cell membranes.

Synonym: SPARC, Basement-Membrane Protein 40, BM-40, Osteonectin, ON, Secreted Protein Acidic and Rich in Cysteine, SPARC, ON

Poids moléculaire: 33.7 kDa

UniProt: P09486

Pathways: Autophagy