P-Selectin Protein (His tag)
-
- Antigène Voir toutes P-Selectin (SELP) Protéines
- P-Selectin (SELP) (Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP))
- Type de proteíne
- Recombinant
- Activité biologique
- Active
-
Origine
- Souris
-
Source
- HEK-293 Cells
- Purification/Conjugué
- Cette P-Selectin protéine est marqué à la His tag.
- Fonction
- Recombinant Mouse SELP/selectin P/P-selectin Protein (His Tag)(Active)
- Séquence
- Met1-Ala709
- Attributs du produit
- A DNA sequence encoding the mouse SELP (Q01102) (Met1-Ala709) was expressed with a C-terminal polyhistidine tag.
- Pureté
- > 95 % as determined by SDS-PAGE
- niveau d'endotoxine
- < 1.0 EU per μg of the protein as determined by the LAL method.
- Biological Activity Comment
- Measured by the ability of the immobilized protein to support the adhesion of U937 cells.When 5 x 10E4 cells/well are added to SELP-coated plates (5 μg/mL and 100 μL/well), approximately >70% cells will adhere specifically after 60 minutes at 37°C.
- Top Product
- Discover our top product SELP Protéine
-
-
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Reconstitution
- Please refer to the printed manual for detailed information.
- Buffer
- Lyophilized from sterile PBS, pH 7.4
- Stock
- 4 °C,-20 °C,-80 °C
- Stockage commentaire
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
-
- Antigène
- P-Selectin (SELP) (Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP))
- Autre désignation
- SELP/selectin P/P-selectin (SELP Produits)
- Synonymes
- PSELECT Protein, CD62 Protein, CD62P Protein, GMP140 Protein, GRMP Protein, LECAM3 Protein, PADGEM Protein, PSEL Protein, GMP-140 Protein, Grmp Protein, selectin P Protein, selectin, platelet Protein, Selp Protein, SELP Protein
- Sujet
-
Background: P selectin (SELP) is a 140 kDa protein that is stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. SELP mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with PSGL1. P selectin is a cell adhesion molecule on the surface of activated endothelial cells. Cellular adhesion molecules are a large family of proteins that attach the cytoskeleton and intracellular signaling cascades with the extracellular environment. SELP is a calcium-dependent receptor for myeloid cells that binds to sialylated forms of Lewis blood group carbohydrate antigens on neutrophils and monocytes. This protein redistributes to the plasma membrane during platelet activation and degranulation and mediates the interacton of activated endothelial cells or platelets with leukocytes.
Synonym: CD62P,GMP-140,Grmp,LECAM3,PADGEM
- Poids moléculaire
- 74 kDa
- UniProt
- Q01102
-