SMPD1 Protein (AA 1-631) (His tag)

N° du produit ABIN7198101

Détail du produit

Antigène: SMPD1 (Sphingomyelin phosphodiesterase 1, Acid Lysosomal (SMPD1))

Type de proteíne: Recombinant

Activité biologique: Active

Attributs du protein: AA 1-631

Origine: Humain

Source: Baculovirus infected Insect Cells

Purification/Conjugué: Cette SMPD1 protéine est marqué à la His tag.

Fonction: Recombinant Human SMPD1/ASM Protein (aa 1-631, His Tag)(Active)

Séquence: Met 1-Cys 631

Attributs du produit: A DNA sequence encoding the full length of human SMPD1 isoform 1 (NP_000534.3) (Met 1-Cys 631) was expressed, fused with a polyhistidine tag at the C-terminus.

Pureté: > 94 % as determined by reducing SDS-PAGE.

niveau d'endotoxine: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: Measured by its ability to cleave. 2-N-Hexadecanoylamino-4-nitrophenylphosphorylcholine (HNPPC). The specific activity is >1,000 pmol/min/μg.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 50 mM Tris, 100 mM NaCl, pH 8.0, 0.1 % OGP, 10 % glycerol

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Détail du antigène

Antigène: SMPD1 (Sphingomyelin phosphodiesterase 1, Acid Lysosomal (SMPD1))

Autre désignation: SMPD1/ASM (SMPD1 Produits)

Synonymes: ASM Protein, ASMASE Protein, NPD Protein, A-SMase Protein, Zn-SMase Protein, aSMase Protein, SMPD1 Protein, sphingomyelin phosphodiesterase 1 Protein, sphingomyelin phosphodiesterase 1, acid lysosomal Protein, sphingomyelin phosphodiesterase Protein, SMPD1 Protein, Smpd1 Protein, LOC5578088 Protein

Sujet:

Background: Sphingomyelin phosphodiesterase 1 (SMPD1) , also known as ASM ( acid sphingomyelinase ), is a member of the acid sphingomyelinase family of enzymes. Three isoforms have been identified, isoform 1 is 631 amino acids (aa) in length as the pro form, while Isoform 2 and isoform 3 have lost catalytic activity. The active SMPD1 isoform 1 contains one saposin B-type domain that likely interacts with sphingomyelin, and a catalytic region. Human SMPD1 is 86 % aa identical to mouse SMPD1. SMPD1 is a monomeric lysosomal enzyme that converts sphingomyelin (a plasma membrane lipid ) into ceramide through the removal of phosphorylcholine. This generates second messenger components that participate in signal transduction. Defects in SMPD1 are the cause of Niemann-Pick disease type A (NPA) and type B (NPB), also known as Niemann-Pick disease classical infantile form and Niemann-Pick disease visceral form. Niemann-Pick disease is a clinically and genetically heterogeneous recessive disorder. NPB has little if any neurologic involvement and patients may survive into adulthood.

Synonym: ASM,ASMASE,NPD

Poids moléculaire: 65 kDa

NCBI Accession: NP_000534