Dnmt2 Protein (GST tag)

N° du produit ABIN7317610

Détail du produit

Antigène: Dnmt2 (TRDMT1) (tRNA Aspartic Acid Methyltransferase 1 (TRDMT1))

Type de proteíne: Recombinant

Origine: Humain

Source: Baculovirus infected Insect Cells

Purification/Conjugué: Cette Dnmt2 protéine est marqué à la GST tag.

Fonction: Recombinant Human DNMT2/TRDMT1 Protein (GST Tag)

Séquence: Met 1-Glu 391

Attributs du produit: A DNA sequence encoding the human TRDMT1 isoform a (NP_004403.1) (Met 1-Glu 391) was fused with the GST tag at the N-terminus.

Pureté: > 94 % as determined by reducing SDS-PAGE.

niveau d'endotoxine: < 1.0 EU per μg as determined by the LAL method.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 50 mM Tris, 100 mM NaCl, 0.5 mM GSH, 0.5 mM PMSF, pH 8.0

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Détail du antigène

Antigène: Dnmt2 (TRDMT1) (tRNA Aspartic Acid Methyltransferase 1 (TRDMT1))

Autre désignation: DNMT2/TRDMT1 (TRDMT1 Produits)

Synonymes: MGC89267 Protein, DMNT2 Protein, DNMT2 Protein, MHSAIIP Protein, PUMET Protein, RNMT1 Protein, Dnmt2 Protein, Rnmt2 Protein, dnmt2 Protein, tRNA aspartic acid methyltransferase 1 Protein, tRNA aspartic acid methyltransferase 1 L homeolog Protein, trdmt1 Protein, TRDMT1 Protein, Trdmt1 Protein, trdmt1.L Protein

Sujet:

Background: DNMT2, also known as tRNA (cytosine-5-)-methyltransferase, DNA methyltransferase homolog HsaIIP, and TRDMT1, is a member of the DNA methyltransferase family of enzymes. DNMT2 enzymes have been widely conserved during evolution and contain all of the signature motifs of DNA (cytosine-5)-methyltransferases. It contains all 10 sequence motifs that are conserved among m(5)C MTases, including the consensus S:-adenosyl-L-methionine-binding motifs and the active site ProCys dipeptide, and its structure is very similar to prokaryotic DNA methyltransferases. DNMT2 has close homologs in plants, insects and Schizosaccharomyces pombe, but no related sequence can be found in the genomes of Saccharomyces cerevisiae or Caenorhabditis elegans. While the biological function of DNMT2 is not yet known, the strong binding to DNA suggests that DNMT2 may mark specific sequences in the genome by binding to DNA through the specific target-recognizing motif. However, the DNA methyltransferase activity of these proteins is comparatively weak and their biochemical and functional properties remain enigmatic. Recent evidence now shows that Dnmt2 has a novel tRNA methyltransferase activity, raising the possibility that the biological roles of these proteins might be broader than previously thought.

Synonym: DMNT2,DNMT2,MHSAIIP,PUMET,RNMT1

Poids moléculaire: 71 kDa

NCBI Accession: NP_004403