TPSB2 Protein (His tag)

N° du produit ABIN7320763

Détail du produit

Antigène: TPSB2 (Tryptase beta 2 (TPSB2))

Type de proteíne: Recombinant

Origine: Souris

Source: Human Cells

Purification/Conjugué: Cette TPSB2 protéine est marqué à la His tag.

Fonction: Recombinant Mouse MCPT6/Mast Cell Protease-6 Protein (His Tag)

Séquence: Ala23-Ser276

Attributs du produit: Recombinant Mouse MCPT6 is produced by our Mammalian expression system and the target gene encoding Ala23-Ser276 is expressed with a 6His tag at the C-terminus.

Pureté: > 95 % as determined by reducing SDS-PAGE.

niveau d'endotoxine: < 1.0 EU per μg as determined by the LAL method.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Frozen, Liquid

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM Tris-HCl,150 mMNacl, pH 7.5.

Stock: -20 °C

Stockage commentaire: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Détail du antigène

Antigène: TPSB2 (Tryptase beta 2 (TPSB2))

Autre désignation: MCPT6/Mast Cell Protease-6 (TPSB2 Produits)

Synonymes: TPS2 Protein, tryptaseB Protein, tryptaseC Protein, Mcpt6 Protein, tryptase beta 2 (gene/pseudogene) Protein, tryptase beta 2 Protein, TPSB2 Protein, Tpsb2 Protein

Sujet:

Background: Tryptase beta-2(Tpsb2), also known as Mast cell protease 6( mMCP-6), belongs to the peptidase S1 family and Tryptase subfamily. Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. It plays a role in innate immunity. Tpsb2 can be detected primarily in skin during embryogenesis. Tpsb2 can not be detected at early embryonic stages but is abundantly expressed in later stages with a peak at E17.5-E18.5. Tryptase is a homotetramer. The active tetramer is converted to inactive monomers at neutral and acidic pH in the absence of heparin. Low concentrations of inactive monomers become active monomers at pH 6.0 in the presence of heparin. When the concentration of active monomers is higher, they convert to active monomers and then to active tetramers. These monomers are active and functionally distinct from the tetrameric enzyme. In contrast to the hidden active sites in the tetrameric form, the active site of the monomeric form is accessible for macromolecular proteins and inhibitors eg: fibrinogen which is a substrate for the monomeric but not for the tetrameric form. The monomeric form forms a complex with SERPINB6.

Synonym: Tryptase beta-2, Tryptase-2, Mast cell protease 6, mMCP-6, Tpsb2

Poids moléculaire: 29.3 kDa

UniProt: P21845