TNK2 Protein (GST tag)

N° du produit ABIN7317144

Protéine Recombinant TNK2 exprimée dans Baculovirus infected Insect Cells.

Aperçu rapide pour TNK2 Protein (GST tag) (ABIN7317144)

Antigène: TNK2 (tyrosine Kinase, Non-Receptor, 2 (TNK2))

Type de proteíne: Recombinant

Origine: Humain

Source: Baculovirus infected Insect Cells

Pureté: > 90 % as determined by reducing SDS-PAGE.

Détail du produit

Purification/Conjugué: Cette TNK2 protéine est marqué à la GST tag.

Fonction: Recombinant Human ACK1/TNK2 Protein (GST Tag)

Séquence: Gly 110-Trp 476

Attributs du produit: A DNA sequence encoding the amino acid (Gly 110-Trp 476) of human ACK1 isoform 1 (NP_005772.3) was expressed with the GST tag at the N-terminus.

niveau d'endotoxine: < 1.0 EU per μg as determined by the LAL method.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Frozen, Liquid

Buffer: Supplied as sterile 20 mM Tris, 500 mM NaCl, pH 7.4, 10 % glycerol, 0.5 mM EDTA, 0.5 mM PMSF, 0.5 mM TCEP

Stock: -20 °C

Stockage commentaire: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Détail du antigène

Antigène: TNK2 (tyrosine Kinase, Non-Receptor, 2 (TNK2))

Autre désignation: ACK1/TNK2

Sujet:

Background: ACK1 (also known as ACK, TNK2, or activated Cdc42 kinase) is a structurally unique non-receptor tyrosine kinase that is expressed in diverse cell types. This downstream effector of CDC42 which mediates CDC42-dependent cell migration via phosphorylation of BCAR1. The ACK1 protein may be involved in a regulatory mechanism that sustains the GTP-bound active form of Cdc42Hs and which is directly linked to a tyrosine phosphorylation signal transduction pathway. ACK1 integrates signals from plethora of ligand-activated receptor tyrosine kinases (RTKs), for example, MERTK, EGFR, HER2 and PDGFR to initiate intracellular signaling cascades. It binds to both poly- and mono-ubiquitin and regulates ligand-induced degradation of EGFR. ACK1 transduces extracellular signals to cytosolic and nuclear effectors such as the protein kinase AKT/PKB and androgen receptor (AR), to promote cell survival and growth. ACK1 participates in tumorigenesis, cell survival, and migration. Gene amplification and overexpression of ACK1 were found in many cancer types such as those of the lung and prostate. Recently, four somatic missense mutations of ACK1, which occur in the N-terminal region, the C-lobe of the kinase domain, and the SH3 domain, were identified in cancer tissue samples.

Synonym: ACK,ACK-1,ACK1,p21cdc42Hs

Poids moléculaire: 68 kDa

NCBI Accession: NP_005772