UBE2F Protein (His tag)

N° du produit ABIN7198582

Détail du produit

Antigène: UBE2F (Ubiquitin-Conjugating Enzyme E2F (UBE2F))

Type de proteíne: Recombinant

Origine: Humain

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette UBE2F protéine est marqué à la His tag.

Fonction: Recombinant Human UBE2F Protein (His Tag)

Séquence: Met 1-Arg 185

Attributs du produit: A DNA sequence encoding the human UBE2F isoform 1 (Q969M7-1) (Met 1-Arg 185) was expressed, with a polyhistide tag at the N-terminus.

Pureté: > 94 % as determined by reducing SDS-PAGE.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, 10 % glycerol, 2 mM DTT, pH 7.5

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Détail du antigène

Antigène: UBE2F (Ubiquitin-Conjugating Enzyme E2F (UBE2F))

Autre désignation: UBE2F (UBE2F Produits)

Synonymes: NCE2 Protein, 2510010F15Rik Protein, AI851109 Protein, RGD1307608 Protein, zgc:77005 Protein, ubiquitin conjugating enzyme E2 F (putative) Protein, NEDD8-conjugating enzyme UBE2F Protein, ubiquitin-conjugating enzyme E2F (putative) Protein, ubiquitin conjugating enzyme E2F (putative) S homeolog Protein, UBE2F Protein, Tsp_01616 Protein, ube2f Protein, ube2f.S Protein, Ube2f Protein

Sujet:

Background: UBE2F is a member of the ubiquitin-conjugating E2 family whose members perform the second step in the ubiquitination reaction. Initially identified as the main process for protein degradation, ubiquitination is believed nowadays to be crucial for a wider range of cellular processes. The outcome of the ubiquitin-conjugation reaction, and thereby the fate of the substrate, is heavily dependent on the number of ubiquitin molecules attached and how these ubiquitin molecules are inter-connected. To deal with this complexity and to allow adequate ubiquitination in time and space, a highly sophisticated conjugation machinery has been developed. In a sequential manner, ubiquitin becomes activated by an ubiquitin-activating enzyme (E1), which then transfers the ubiquitin to a group of ubiquitin-conjugating enzymes (E2s). Next, ubiquitin-loaded E2s are interacting with ubiquitin protein ligases (E3s) and ubiquitin is conjugated to substrates on recruitment by the E3. These three key enzymes are operating in a hierarchical system, wherein two E1s and 35 E2s have been found and hundreds of E3s have been identified in humans. 

Synonym: NCE2

Poids moléculaire: 22.9 kDa