ARTN Protein (AA 112-224) (Fc Tag)

N° du produit ABIN7273877

Détail du produit

Antigène: ARTN (Artemin (ARTN))

Type de proteíne: Recombinant

Attributs du protein: AA 112-224

Origine: Souris

Source: HEK-293 Cells

Purification/Conjugué: Cette ARTN protéine est marqué à la Fc Tag.

Fonction: Mouse ARTN Protein

Séquence: Ala112-Gly224

Attributs du produit: Recombinant Mouse ARTN Protein is expressed from HEK293 with hFc tag at the N-Terminus.It contains Ala112-Gly224.

Pureté: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Mouse GFRA3, His Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Mouse ARTN, hFc Tag with the EC50 of 0.12μg/ml determined by ELISA. See testing image for detail.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in 50 mM Glycine,150 mM NaCl ( pH 3.2).

Buffer: Lyophilized from 0.22μm filtered solution in 50 mM Glycine,150 mM NaCl ( pH 3.2). Normally 8 % trehalose is added as protectant before lyophilization.

Stock: -20 °C,-80 °C

Stockage commentaire: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: ARTN (Artemin (ARTN))

Autre désignation: ARTN (ARTN Produits)

Synonymes: ARTN Protein, ENOVIN Protein, EVN Protein, NBN Protein, neublastin Protein, artemin Protein, ARTN Protein, Artn Protein

Sujet: Artemin (ARTN) is a member of glial cell line-derived neurotrophic factor (GDNF) family of ligands, and its signaling is mediated via a multi-component receptor complex including the glycosylphosphatidylinositol-anchored GDNF family receptors a (GFRa1, GFRa3) and RET receptor tyrosine kinase. The major mechanism of ARTN action is via binding to a non-signaling co-receptor. The major function of ARTN is to drive the molecule to induce migration and axonal projection from sympathetic neurons.

Poids moléculaire: 39.4 kDa. Due to glycosylation, the protein migrates to 65-80 kDa based on Tris-Bis PAGE result.