BCMA Protein (AA 1-54) (Biotin,His-Avi Tag)
Aperçu rapide pour BCMA Protein (AA 1-54) (Biotin,His-Avi Tag) (ABIN7273938)
Antigène
Voir toutes BCMA (TNFRSF17) ProtéinesType de proteíne
Activité biologique
Origine
Source
Pureté
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Attributs du protein
- AA 1-54
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Purification/Conjugué
- Cette BCMA protéine est marqué à la Biotin,His-Avi Tag.
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Fonction
- Biotinylated Human BCMA/TNFRSF17 Protein
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Séquence
- Met1-Ala54
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Attributs du produit
- Recombinant Biotinylated Human BCMA/TNFRSF17 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Met1-Ala54.
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Stérilité
- 0.22 μm filtered
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niveau d'endotoxine
- Less than 1 EU per μg by the LAL method.
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Biological Activity Comment
- Immobilized Biotinylated Human BCMA, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human BAFF, hFc Tag with the EC50 of 4.6ng/ml determined by ELISA (QC Test).,Immobilized Human APRIL (Trimer), His Tag at 1ug/ml(100ul/well) on the plate. Dose response curve for Biotinylated Human BCMA, His Tag with the EC50 of 38.2ng/ml determined by ELISA.,Immobilized Anti-BCMA Antibody, hFc Tag at 0.2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human BCMA, His Tag with the EC50 of 3.4ng/ml determined by ELISA.,Biotinylated Human BCMA on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Loaded Anti-BCMA antibody on ProA-Biosensor can bind Biotinylated Human BCMA, His-Avi Tag with an affinity constant of 4.09 nM as determined in BLI assay (Gator® Prime).
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.
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Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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Stock
- -20 °C,-80 °C
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Stockage commentaire
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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Date de péremption
- 12 months
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- BCMA (TNFRSF17) (Tumor Necrosis Factor Receptor Superfamily, Member 17 (TNFRSF17))
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Autre désignation
- BCMA
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Sujet
- B-cell maturation antigen (BCMA or BCM), also known as tumor necrosis factor receptor superfamily member 17 (TNFRSF17), is a protein that in humans is encoded by the TNFRSF17 gene.TNFRSF17 is a cell surface receptor of the TNF receptor superfamily which recognizes B-cell activating factor (BAFF).
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Poids moléculaire
- 8.9 kDa. Due to glycosylation, the protein migrates to 12 kDa and 15-17 kDa based on Bis-Tris PAGE result.
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UniProt
- Q02223
Antigène
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