BCMA Protein (AA 1-54) (Fc Tag,AVI tag,Biotin)

N° du produit ABIN3137676

Détail du produit

Antigène: BCMA (TNFRSF17) (Tumor Necrosis Factor Receptor Superfamily, Member 17 (TNFRSF17))

Type de proteíne: Recombinant

Activité biologique: Active

Attributs du protein: AA 1-54

Origine: Humain

Source: HEK-293 Cells

Purification/Conjugué: Cette BCMA protéine est marqué à la Fc Tag,AVI tag,Biotin.

Marque: MABSol®,PrecisionAvi

Séquence: AA 1-54

Specificité: Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Attributs du produit: This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag™). The protein has a calculated MW of 33.8 kDa. As a result of glycosylation, the protein migrates as 40-45 kDa under reducing (R) condition, and 70-85 kDa under non-reducing (NR) condition (SDS-PAGE).

Pureté: >95 % as determined by SDS-PAGE.

niveau d'endotoxine: Less than 1.0 EU per μg by the LAL method.

Information d'application

Commentaires:

Ready-to-use AvitagTM biotinylated protein:
The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: Tris with Glycine, Arginine and NaCl,  pH 7.5

Conseil sur la manipulation: Please avoid repeated freeze-thaw cycles.

Stock: -20 °C

Référence

Berahovich, Zhou, Xu, Wei, Guan, Guan, Harto, Fu, Yang, Zhu, Li, Wu, Golubovskaya: "CAR-T Cells Based on Novel BCMA Monoclonal Antibody Block Multiple Myeloma Cell Growth." dans: Cancers, Vol. 10, Issue 9, (2018) (PubMed).

Détail du antigène

Antigène: BCMA (TNFRSF17) (Tumor Necrosis Factor Receptor Superfamily, Member 17 (TNFRSF17))

Autre désignation: BCMA (TNFRSF17 Produits)

Synonymes: TNFRSF17 Protein, BCM Protein, BCMA Protein, CD269 Protein, TNFRSF13A Protein, Tnfrsf13 Protein, Tnfrsf13a Protein, TNF receptor superfamily member 17 Protein, tumor necrosis factor receptor superfamily, member 17 Protein, Tnfrsf17 Protein, TNFRSF17 Protein

Sujet: Tumor necrosis factor receptor superfamily member 17 (TNFRSF17) is also known as B-cell maturation protein (BCMA), CD antigen CD269, which is a member of the TNF-receptor superfamily. TNFRSF17 contains one TNFR-Cys repeat. TNFRSF17 is expressed in mature B-cells, but not in T-cells or monocytes. TNFRSF17 is receptor for TNFSF13B/BLyS/BAFF and TNFSF13/APRIL. TNFRSF17 promotes B-cell survival and plays a role in the regulation of humoral immunity. TNFRSF17 can activate NF-kappa-B and JNK.

Poids moléculaire: 33.8 kDa

NCBI Accession: NP_001183