VTCN1 Protein (AA 29-258) (His tag)

N° du produit ABIN7273949

Détail du produit

Antigène: VTCN1 (V-Set Domain Containing T Cell Activation Inhibitor 1 (VTCN1))

Type de proteíne: Recombinant

Attributs du protein: AA 29-258

Origine: Macaque rhésus, Cynomolgus

Source: HEK-293 Cells

Purification/Conjugué: Cette VTCN1 protéine est marqué à la His tag.

Fonction: Cynomolgus/Rhesus macaque B7-H4 Protein

Séquence: Phe29-Ala258

Attributs du produit: Recombinant Cynomolgus/Rhesus macaque B7-H4 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Phe29-Ala258.

Pureté: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Cynomolgus/Rhesus macaque B7-H4, His Tag at 0.2μg/ml (100μl/Well) on the plate. Dose response curve for Anti-B7-H4 Antibody, hFc Tag with the EC50 of 12.8ng/ml determined by ELISA. See testing image for detail.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Stock: -20 °C,-80 °C

Stockage commentaire: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: VTCN1 (V-Set Domain Containing T Cell Activation Inhibitor 1 (VTCN1))

Autre désignation: B7-H4 (VTCN1 Produits)

Synonymes: B7h4 Protein, B7s1 Protein, B7x Protein, BC032925 Protein, B7-H4 Protein, B7H4 Protein, B7S1 Protein, B7X Protein, B7h.5 Protein, PRO1291 Protein, VCTN1 Protein, RGD1311204 Protein, V set domain containing T cell activation inhibitor 1 L homeolog Protein, V-set domain containing T cell activation inhibitor 1 Protein, V-set domain containing T-cell activation inhibitor 1 Protein, V set domain containing T cell activation inhibitor 1 Protein, vtcn1.L Protein, VTCN1 Protein, vtcn1 Protein, Vtcn1 Protein

Sujet: B7-H4, also known as B7x and B7S1, is a 50-80 kDa glycosylated member of the B7 family of immunomodulatory proteins.B7-H4 is up-regulated in several carcinomas in correlation with tumor progression and metastasis. A soluble form of B7-H4 is elevated in the serum of ovarian cancer, renal cell carcinoma, and rheumatoid arthritis patients, also in correlation with advanced disease status .

Poids moléculaire: 26.41 kDa. Due to glycosylation, the protein migrates to 48-63 kDa based on Tris-Bis PAGE result.

UniProt: A0A2K5U6P5, F7B770