CD276 Protein (CD276) (AA 27-461) (Fc Tag)

N° du produit ABIN7273957

Détail du produit

Antigène: CD276

Type de proteíne: Recombinant

Attributs du protein: AA 27-461

Origine: Humain

Source: HEK-293 Cells

Purification/Conjugué: Cette CD276 protéine est marqué à la Fc Tag.

Fonction: Human B7-H3 (4Ig) /B7-H3b Protein

Séquence: Gly27-Thr461

Attributs du produit: Recombinant Human B7-H3 (4Ig)/B7-H3b Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Gly27-Thr461.

Pureté: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human B7-H3 (4Ig) , hFc Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-B7-H3 Antibody, hFc Tag with the EC50 of 44.3ng/ml determined by ELISA. See testing image for detail.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Stock: -20 °C,-80 °C

Stockage commentaire: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: CD276

Autre désignation: B7-H3 (CD276 Produits)

Synonymes: sc:d0645 Protein, zgc:158279 Protein, 6030411F23Rik Protein, AU016588 Protein, B7RP-2 Protein, B7h3 Protein, B7H3 Protein, 4Ig-B7-H3 Protein, B7-H3 Protein, CD276 molecule Protein, CD276 molecule S homeolog Protein, CD276 antigen Protein, Cd276 molecule Protein, CD276 Protein, cd276.S Protein, cd276 Protein, Cd276 Protein

Sujet: B7-H3, a member of the B7 family of immunomodulatory molecules, is overexpressed in a wide range of solid cancers.B7-H3 binds to activated T cells via an as yet unidentified receptor. In assays using sub-optimal amount so anti-CD3 stimulation, 2Ig-B7-H3 enhances T cell proliferation, T cell interferon-gamma (IFN-gamma) production, and cytotoxic T cells induction.

Poids moléculaire: 73.4 kDa. Due to glycosylation, the protein migrates to 80-110 kDa based on Tris-Bis PAGE result.

Pathways: Cancer Immune Checkpoints